Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ABHD5 Double Nickase Plasmid (h): sc-402273-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ABHD5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ABHD5 Double Nickase Plasmid (h) and ABHD5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ABHD5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ABHD5 Antibody (E-1): sc-376931
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ABHD5 Double Nickase Plasmid (h) | sc-402273-NIC | 20 µg | $410.00 | |||
ABHD5 Double Nickase Plasmid (h2) | sc-402273-NIC-2 | 20 µg | $410.00 |
ABHD5 (also known as CGI-58) encodes a lipid droplet–associated coactivator that stimulates adipose triglyceride lipase (PNPLA2/ATGL) and promotes intracellular triglyceride hydrolysis. Through regulation of lipolysis, fatty acid mobilization, and lipid droplet dynamics, ABHD5 influences energy homeostasis and downstream signaling linked to mitochondrial β-oxidation and cellular stress responses. Disruption of ABHD5-dependent lipid catabolism is associated with neutral lipid storage phenotypes and has been implicated in metabolic dysregulation affecting liver, skin, and muscle tissues. As a central node in lipid metabolism, ABHD5 is frequently studied in the context of lipid storage disorders, inflammation-linked metabolic pathways, and cell-type–specific lipid droplet remodeling.
ABHD5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ABHD5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ABHD5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ABHD5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ABHD5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.