ABCG1 CRISPR/Cas9 KO Plasmid (m): sc-418933
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ABCG1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ABCG1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ABCG1 CRISPR/Cas9 KO Plasmid (m) | sc-418933 | 20 µg | $397.00 |
Overview
Abcg1 encodes the ATP-binding cassette transporter ABCG1, a key regulator of intracellular cholesterol and phospholipid homeostasis that promotes sterol efflux to HDL and supports membrane lipid remodeling. In macrophages and other lipid-handling cells, ABCG1 functions downstream of LXR/RXR signaling and coordinates with ABCA1 to control lipid loading, foam cell formation, and inflammatory tone. Altered Abcg1 activity has been linked to dysregulated lipid metabolism, oxidative stress responses, and atherosclerosis-relevant phenotypes in murine models. In addition, ABCG1-mediated lipid balance can influence immune cell function and cellular responses to metabolic stress.
ABCG1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Abcg1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Abcg1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Abcg1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ABCG1 protein expression.
This CRISPR knockout system enables efficient generation of Abcg1-deficient cell models for investigation of ABCG1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Abcg1 exon(s) critical for ABCG1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Abcg1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ABCG1 CRISPR/Cas9 KO Plasmid (m) and ABCG1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Abcg1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ABCG1 HDR Plasmid (m) and ABCG1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Abcg1 homology arms to support homology-directed repair at defined Abcg1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.