ABCA4 Antibody (3F4) recommended for detection of ABCA4 of mouse, rat and human origin by WB, IP, IF and IHC(P)

ABCA4 Antibody (3F4): sc-65672

ABCA4 Antibody (3F4) is rated 3.8 out of 5 by 4.
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Datasheets

    • ABCA4 Antibody (3F4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against the 220-kDa ABCA4 protein of bovine origin
    • Anti-ABCA4 Antibody (3F4) is recommended for detection of ABCA4 of mouse, rat and human origin by WB, IP, IF and IHC(P); also reactive with additional species, including bovine
    • ABCA4 Antibody (3F4) is available conjugated to agarose for IP; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ABCA4 (3F4): sc-65672.

I purchased your ABCA4 (3F4) sc-65672 antibody last year and tried it on fibroblast cells following one of the published paper but I didn't get the expected band. Can you help me with that? Thanks.

Asked by: Di Huang
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-09-11

Hello I wish to know if it is possible to recieve the 3F4 ABCA4 antibody conjugated to agarose or sepharose. And if so how the 3F4-immunoaffinity column should be prepared.

Asked by: Tess555
Thank you for your question. We provide sc-65672 conjugated to agarose for IP experiments. If you require more information, please contact our Technical Service https://www.scbt.com/scbt/resources/technical-service
Answered by: SCBT Heidelberg Support
Date published: 2018-12-22

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with ABCA4 Antibody (3F4): sc-65672?

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 4
Date published: 2017-01-09
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Rated 3 out of 5 by from Not so good as other ABC family antibody The band is not clear enough, there are many inspecialized bands on the film.
Date published: 2018-01-30
Rated 4 out of 5 by from Nice western Very clean western blot data; would like to see staining
Date published: 2017-01-10
Rated 5 out of 5 by from Reliable and Versatile Antibody Strong, clear signal in WB with little to no background. Very Reliable. Works well in a variety of species including mouse, rat, bovine, and human samples,
Date published: 2016-12-22
Rated 3 out of 5 by from Light but positive band in WB in mouse eye Light but positive band in WB in mouse eye tissue extract. -SCBT QC
Date published: 2014-01-09
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