ABC1 Double Nickase Plasmid (h): sc-401086-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ABC1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ABC1 Double Nickase Plasmid (h) and ABC1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ABCA1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ABC1 Antibody (AC10): sc-53482
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ABC1 Double Nickase Plasmid (h) | sc-401086-NIC | 20 µg | $410.00 | |||
ABC1 Double Nickase Plasmid (h2) | sc-401086-NIC-2 | 20 µg | $410.00 |
ABCA1 (ABC1) is an ATP-binding cassette transporter that mediates cellular cholesterol and phospholipid efflux to apolipoprotein A-I, supporting nascent HDL formation and reverse cholesterol transport. By regulating lipid homeostasis, ABCA1 influences membrane composition, lipid raft organization, and downstream signaling processes linked to macrophage cholesterol handling and inflammatory responses. ABCA1 activity intersects with LXR/RXR-regulated transcriptional programs and broader pathways controlling sterol sensing and lipoprotein metabolism. Genetic or functional perturbation of ABCA1 is associated with dyslipidemia phenotypes and altered atherogenesis-related biology, making it a key target for mechanistic studies of lipid transport and cardiometabolic disease pathways.
ABC1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ABCA1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ABCA1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ABCA1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ABCA1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.