Anti-ABC1 Antibody (AC10) recommended for detection of ABC1 of mouse, rat and human origin by WB, IP and IF

Anti-ABC1 Antibody (AC10): sc-53482

Anti-ABC1 Antibody (AC10) is rated 4.5 out of 5 by 2.
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Datasheets

    • ABC1 Antibody (AC10) is a mouse monoclonal IgG1 κ, cited in 7 publications, provided at 200 µg/ml
    • raised against amino acids 1874-2261 mapping near the C-terminus of ABC1 of human origin
    • Anti-ABC1 Antibody (AC10) is recommended for detection of ABC1 of mouse, rat and human origin by WB, IP and IF
    • Anti-ABC1 Antibody (AC10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ABC1 (AC10): sc-53482.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for ABC1 Antibody (AC10) for WB applications. These reagents are now offered in bundles with ABC1 Antibody (AC10) (see ordering information below).
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The protein size seems incorrect, ABCA1 should be 254kDa

Asked by: linnn
Thank you for your question. The expected molecular weight for ABC1 is between 220-250kDa. Please reach out to Technical service or your local Distributor if you have any further questions.
Answered by: Tech Service
Date published: 2019-08-28

Are there any issues with using sc-53482: ABC1 (AC10) mouse monoclonal primary antibody on mouse samples?

Asked by: Germaine
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-53482 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by from Great This is an excellent antibod, I suggest use dilution 1:500 and the band is pretty clear.
Date published: 2017-09-12
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