Each CRISPR/Cas9 Knockout (KO) Plasmid product consists of a pool of 3 plasmids designed to ensure identification and cleavage of a specific gene for maximum knockout efficiency. Each plasmid encodes a unique 20 nt sequence derived from the GeCKO (v2) library.
53BP1 CRISPR Plasmids (m)
- Target species: mouse; for corresponding human product, see 53BP1 CRISPR Plasmids (h)
- CRISPR/Cas9 KO Plasmids consists of 53BP1-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library
- For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break (DSB) in the genomic DNA
- Target-specific CRISPR Plasmids for both gene knockout and activation are available. Please refer to the detailed product information in the tabs below
- Gene knockdown or activation can be assayed using p-53BP1 Antibody (38.Ser 25): sc-135748
- All products are provided as transfection-ready, purified plasmid DNA, except the Lentiviral Activation Particles which have been prepackaged as Lentiviral Particles for use with hard-to-transfect cells
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CRISPR/Cas9 KO Plasmid
SEE ALSO...
53BP1 Antibodies for analysis of cellular responses to 53BP1 CRISPR Products
Learn more about the CRISPR System
CRISPR/Cas9 KO plasmids, Double Nickase Plasmids and CRISPR/dCas9 Activation plasmids are considered a "Licensed Product" and are to be used in accordance with the Limited/Label License. Click here for details
Description
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- 53BP1 CRISPR/Cas9 Knockout (KO) Plasmid (m2) consists of a pool of three plasmids each encoding the Cas9 nuclease and a 53BP1-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency
- gRNA sequences are derived from the GeCKO (v2) library and direct the Cas9 protein to induce a site-specific double strand break (DSB) in the genomic DNA
- For selection of cells containing a successful Cas9-induced DSB, co-transfection with 53BP1 HDR Plasmid (m2) (sc-424212-HDR-2) is recommended
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: p-53BP1 Antibody (38.Ser 25): sc-135748
See Protocol Online.
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Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
53BP1 CRISPR/Cas9 KO Plasmid (m2) | sc-424212-KO-2 | 20 µg | $397.00 |