4E-BP2 CRISPR/Cas9 KO Plasmid (m): sc-420150
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- 4E-BP2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the 4E-BP2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
4E-BP2 CRISPR/Cas9 KO Plasmid (m) | sc-420150 | 20 µg | $397.00 |
Overview
Eif4ebp2 encodes 4E-BP2, a phosphorylation-sensitive translational repressor that binds eIF4E and modulates assembly of the eIF4F cap-dependent translation initiation complex. As a downstream effector of the PI3K–AKT–mTOR signaling axis, 4E-BP2 integrates nutrient, growth factor, and stress cues to tune global and mRNA-selective translation. In mouse tissues, 4E-BP2 contributes to proteostasis and activity-dependent protein synthesis programs, linking translational control to cellular growth, metabolic adaptation, and differentiation. Dysregulated mTOR–4E-BP signaling and aberrant cap-dependent translation are frequently associated with proliferative and neurobiological phenotypes, making Eif4ebp2 a useful node for mechanistic studies of translational control.
4E-BP2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Eif4ebp2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Eif4ebp2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Eif4ebp2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish 4E-BP2 protein expression.
This CRISPR knockout system enables efficient generation of Eif4ebp2-deficient cell models for investigation of 4E-BP2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Eif4ebp2 exon(s) critical for 4E-BP2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Eif4ebp2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by 4E-BP2 CRISPR/Cas9 KO Plasmid (m) and 4E-BP2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Eif4ebp2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by 4E-BP2 HDR Plasmid (m) and 4E-BP2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Eif4ebp2 homology arms to support homology-directed repair at defined Eif4ebp2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.