2B28 CRISPR/Cas9 KO Plasmid (m): sc-432576
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- 2B28 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the 2B28 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
2B28 CRISPR/Cas9 KO Plasmid (m) | sc-432576 | 20 µg | $397.00 |
Overview
Ubxn1 (also referred to as 2B28) encodes a UBX domain–containing adaptor protein that links ubiquitin-dependent processes to the AAA+ ATPase p97/VCP, coordinating substrate extraction and turnover. Through this coupling, UBXN1 contributes to proteostasis control, including endoplasmic reticulum–associated degradation (ERAD), regulation of protein quality control, and modulation of stress-responsive signaling. UBXN1 has been implicated in pathways that influence cell-cycle progression and the handling of misfolded or aggregation-prone proteins, connecting it to mechanisms relevant to neurodegeneration and cancer biology. In mouse systems, perturbing Ubxn1 provides a tractable approach to interrogate p97/VCP-centered networks and ubiquitin-mediated remodeling of protein complexes.
2B28 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ubxn1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ubxn1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ubxn1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish 2B28 protein expression.
This CRISPR knockout system enables efficient generation of Ubxn1-deficient cell models for investigation of 2B28 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ubxn1 exon(s) critical for 2B28 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ubxn1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by 2B28 CRISPR/Cas9 KO Plasmid (m) and 2B28 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ubxn1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by 2B28 HDR Plasmid (m) and 2B28 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ubxn1 homology arms to support homology-directed repair at defined Ubxn1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.