26S Proteasome p50 Antibody (112): sc-65745

26S Proteasome p50 Antibody (112) is a mouse monoclonal IgG2b antibody that detects 26S Proteasome p50 protein of Drosophila melanogaster origin by western blotting (WB) and immunoprecipitation (IP). Anti-26S Proteasome p50 antibody (112) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. 26S proteasome is a crucial multi-subunit complex responsible for degrading ubiquitinated proteins, which maintains cellular homeostasis and regulates various biological processes such as cell cycle, apoptosis, and responses to oxidative stress. 26S proteasome p50, also known as Rpt5 in yeast and S6′ in humans, is one of six ATPase base subunits of 19S regulatory complex, which plays a pivotal role in recognizing ubiquitinated substrates, removing ubiquitin chains, and facilitating protein translocation into 20S core for degradation. This interaction is vital for proteolytic signal recognition, ensuring damaged or misfolded proteins are efficiently processed, preventing potential cellular dysfunction. Assembly of 26S proteasome is ATP-dependent, and due to large size, can disassociate into subcomplexes, including 20S core and two 19S regulatory complexes, highlighting dynamic nature of this essential proteolytic machinery.

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26S Proteasome p50 Antibody (112) References:

1. Assembly of the Drosophila 26 S proteasome is accompanied by extensive subunit rearrangements.  |  Kurucz, E., et al. 2002. Biochem J. 365: 527-36. PMID: 11945175
2. A proteasomal ATPase subunit recognizes the polyubiquitin degradation signal.  |  Lam, YA., et al. 2002. Nature. 416: 763-7. PMID: 11961560
3. The HALTED ROOT gene encoding the 26S proteasome subunit RPT2a is essential for the maintenance of Arabidopsis meristems.  |  Ueda, M., et al. 2004. Development. 131: 2101-11. PMID: 15073153
4. ATP hydrolysis-dependent disassembly of the 26S proteasome is part of the catalytic cycle.  |  Babbitt, SE., et al. 2005. Cell. 121: 553-565. PMID: 15907469
5. The proteasome regulatory particle alters the SAGA coactivator to enhance its interactions with transcriptional activators.  |  Lee, D., et al. 2005. Cell. 123: 423-36. PMID: 16269334
6. The proteasome restricts permissive transcription at tissue-specific gene loci in embryonic stem cells.  |  Szutorisz, H., et al. 2006. Cell. 127: 1375-88. PMID: 17190601
7. Mass spectrometric characterization of the affinity-purified human 26S proteasome complex.  |  Wang, X., et al. 2007. Biochemistry. 46: 3553-65. PMID: 17323924
8. Aging perturbs 26S proteasome assembly in Drosophila melanogaster.  |  Vernace, VA., et al. 2007. FASEB J. 21: 2672-82. PMID: 17413001
9. Rpn13p and Rpn14p are involved in the recognition of ubiquitinated Gcn4p by the 26S proteasome.  |  Seong, KM., et al. 2007. FEBS Lett. 581: 2567-73. PMID: 17499717
10. Prognostic Implication and Immunological Role of PSMD2 in Lung Adenocarcinoma.  |  Zhao, H. and Lu, G. 2022. Front Genet. 13: 905581. PMID: 35754829
11. PSMD2 promotes the progression of bladder cancer and is correlated with immune infiltration.  |  Wang, S., et al. 2022. Front Oncol. 12: 1058506. PMID: 36505799
12. PSMD2 contributes to the progression of esophageal squamous cell carcinoma by repressing autophagy.  |  Liu, Y., et al. 2023. Cell Biosci. 13: 67. PMID: 36998052

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-26S Proteasome p50 Antibody (112) has 2 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

26S Proteasome p50 Antibody (112) Specifications

Alternate Product Name: 26S Proteasome p50

Product Clone Name: 112

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b

Amino Acids:

Epitope Target: 26S Proteasome

Epitope Species: Drosophila melanogaster

Product Reactivity/Detection: p50 subunit of the 19S regulatory base complex of the 26S Proteasome

Species Reactivity/Detection: d

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG_2b in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for 26S Proteasome p50 Antibody (112): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin