26S Proteasome p42E Antibody (IH12): sc-65757
- 26S Proteasome p42E Antibody (IH12) is a mouse monoclonal IgG1 κ 26S Proteasome p42E antibody provided at 200 µg/ml
- raised against 26S Proteasome purified from embryos of Drosophila melanogaster origin
- 26S Proteasome p42E Antibody (IH12) is recommended for detection of p42E subunit of the 19S regulatory base complex of the 26S Proteasome of d origin by WB and IP
- Anti-26S Proteasome p42E Antibody (IH12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for 26S Proteasome p42E Antibody (IH12) for WB applications. These reagents are now offered in bundles with 26S Proteasome p42E Antibody (IH12) (see ordering information below).
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26S Proteasome p42E Antibody (IH12) is a mouse monoclonal IgG1 kappa light chain antibody that detects 26S Proteasome p42E of Drosophila melanogaster origin by western blotting (WB) and immunoprecipitation (IP). Anti-26S Proteasome p42E antibody (IH12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. 26S proteasome is a crucial multi-subunit complex responsible for degrading ubiquitinated proteins, a process vital for maintaining cellular homeostasis and regulating various biological pathways, including cell cycle, apoptosis, and responses to oxidative stress. 26S proteasome consists of over 30 distinct subunits, with p42E being an integral part of 19S regulatory complex, which is essential for recognizing and processing ubiquitinated substrates. This complex not only removes ubiquitin chains but also facilitates substrate translocation into 20S core for degradation. Assembly of 26S proteasome is ATP-dependent, and due to large size, prone to disassociation into smaller subcomplexes, which can impact functionality. Understanding p42E subunit role is critical for elucidating mechanisms of protein regulation and degradation in eukaryotic cells, making 26S Proteasome p42E (IH12) antibody a valuable tool for researchers studying proteasomal function and disease implications.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
26S Proteasome p42E Antibody (IH12) References:
- Assembly of the Drosophila 26 S proteasome is accompanied by extensive subunit rearrangements. | Kurucz, E., et al. 2002. Biochem J. 365: 527-36. PMID: 11945175
- Deletion of proteasomal subunit S5a/Rpn10/p54 causes lethality, multiple mitotic defects and overexpression of proteasomal genes in Drosophila melanogaster. | Szlanka, T., et al. 2003. J Cell Sci. 116: 1023-33. PMID: 12584246
- The HALTED ROOT gene encoding the 26S proteasome subunit RPT2a is essential for the maintenance of Arabidopsis meristems. | Ueda, M., et al. 2004. Development. 131: 2101-11. PMID: 15073153
- ATP hydrolysis-dependent disassembly of the 26S proteasome is part of the catalytic cycle. | Babbitt, SE., et al. 2005. Cell. 121: 553-565. PMID: 15907469
- The proteasome regulatory particle alters the SAGA coactivator to enhance its interactions with transcriptional activators. | Lee, D., et al. 2005. Cell. 123: 423-36. PMID: 16269334
- Mass spectrometric characterization of the affinity-purified human 26S proteasome complex. | Wang, X., et al. 2007. Biochemistry. 46: 3553-65. PMID: 17323924
- Aging perturbs 26S proteasome assembly in Drosophila melanogaster. | Vernace, VA., et al. 2007. FASEB J. 21: 2672-82. PMID: 17413001
- Rpn13p and Rpn14p are involved in the recognition of ubiquitinated Gcn4p by the 26S proteasome. | Seong, KM., et al. 2007. FEBS Lett. 581: 2567-73. PMID: 17499717
- Automated cryoelectron microscopy of 'single particles' applied to the 26S proteasome. | Nickell, S., et al. 2007. FEBS Lett. 581: 2751-6. PMID: 17531228
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
26S Proteasome p42E Antibody (IH12) | sc-65757 | 200 µg/ml | $322.00 | |||
26S Proteasome p42E Antibody (IH12): m-IgG Fc BP-HRP Bundle | sc-526967 | 200 µg Ab; 10 µg BP | $361.00 | |||
26S Proteasome p42E Antibody (IH12): m-IgG1 BP-HRP Bundle | sc-532340 | 200 µg Ab; 20 µg BP | $361.00 | |||
26S Proteasome p42E Antibody (IH12) AC | sc-65757 AC | 500 µg/ml, 25% agarose | $424.00 | |||
26S Proteasome p42E Antibody (IH12) HRP | sc-65757 HRP | 200 µg/ml | $322.00 | |||
26S Proteasome p42E Antibody (IH12) FITC | sc-65757 FITC | 200 µg/ml | $336.00 | |||
26S Proteasome p42E Antibody (IH12) PE | sc-65757 PE | 200 µg/ml | $349.00 | |||
26S Proteasome p42E Antibody (IH12) Alexa Fluor® 488 | sc-65757 AF488 | 200 µg/ml | $364.00 | |||
26S Proteasome p42E Antibody (IH12) Alexa Fluor® 546 | sc-65757 AF546 | 200 µg/ml | $364.00 | |||
26S Proteasome p42E Antibody (IH12) Alexa Fluor® 594 | sc-65757 AF594 | 200 µg/ml | $364.00 | |||
26S Proteasome p42E Antibody (IH12) Alexa Fluor® 647 | sc-65757 AF647 | 200 µg/ml | $364.00 | |||
26S Proteasome p42E Antibody (IH12) Alexa Fluor® 680 | sc-65757 AF680 | 200 µg/ml | $364.00 | |||
26S Proteasome p42E Antibody (IH12) Alexa Fluor® 790 | sc-65757 AF790 | 200 µg/ml | $364.00 |