20S Proteasome β2 Antibody (MCP165): sc-58410

20S Proteasome β2 Antibody (MCP165) is a mouse monoclonal IgG1 antibody that detects 20S Proteasome β2 (PSMB2) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-PSMB2 antibody (MCP165) is offered in a non-conjugated form, allowing for versatile experimental setups. PSMB2 plays a crucial role in cellular protein homeostasis by degrading unnecessary or damaged proteins, a process vital for maintaining cellular function and regulating various biological pathways. The structure of PSMB2 is particularly important, as it consists of a cylindrical core made up of four stacked rings, with each ring containing seven subunits. The outer rings are composed of α subunits that serve as structural components and docking sites for regulatory particles, while the inner rings consist of β subunits that possess catalytic activity essential for protein degradation. This unique architecture facilitates the selective entry of substrates and ensures tightly regulated degradation, preventing uncontrolled proteolysis that could lead to cellular dysfunction. PSMB2 (MCP165) antibody enables researchers to gain insights into proteasome activity and its implications in various diseases, including cancer and neurodegenerative disorders.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

20S Proteasome β2 Antibody (MCP165) References:

1. Eukaryotic 20S proteasome catalytic subunit propeptides prevent active site inactivation by N-terminal acetylation and promote particle assembly.  |  Arendt, CS. and Hochstrasser, M. 1999. EMBO J. 18: 3575-85. PMID: 10393174
2. Primary structural features of the 20S proteasome subunits of rice (Oryza sativa).  |  Sassa, H., et al. 2000. Gene. 250: 61-6. PMID: 10854779
3. Catalytic site-specific inhibition of the 20S proteasome by 4-hydroxynonenal.  |  Ferrington, DA. and Kapphahn, RJ. 2004. FEBS Lett. 578: 217-23. PMID: 15589823
4. Optimization of subsite binding to the beta5 subunit of the human 20S proteasome using vinyl sulfones and 2-keto-1,3,4-oxadiazoles: syntheses and cellular properties of potent, selective proteasome inhibitors.  |  Rydzewski, RM., et al. 2006. J Med Chem. 49: 2953-68. PMID: 16686537
5. Decreased activity of the 20S proteasome in the brain white matter and gray matter of patients with multiple sclerosis.  |  Zheng, J. and Bizzozero, OA. 2011. J Neurochem. 117: 143-53. PMID: 21235577
6. Role of the ubiquitin-proteasome system in the sarcopenic-like phenotype induced by CCL5/RANTES.  |  Conejeros-Lillo, S., et al. 2024. Eur J Transl Myol. 34: PMID: 38357936
7. Ordered structure of the crystallized bovine 20S proteasome.  |  Morimoto, Y., et al. 1995. J Biochem. 117: 471-4. PMID: 7629009
8. Conformational constraints in protein degradation by the 20S proteasome.  |  Wenzel, T. and Baumeister, W. 1995. Nat Struct Biol. 2: 199-204. PMID: 7773788
9. Structure and structure formation of the 20S proteasome.  |  Schmidt, M., et al. 1997. Mol Biol Rep. 24: 103-12. PMID: 9228290
10. Human proteasome subunits from 2-dimensional gels identified by partial sequencing.  |  Kristensen, P., et al. 1994. Biochem Biophys Res Commun. 205: 1785-9. PMID: 7811265
11. Comprehensive mass spectrometric analysis of the 20S proteasome complex.  |  Huang, L. and Burlingame, AL. 2005. Methods Enzymol. 405: 187-236. PMID: 16413316
12. Role of the beta1 subunit in the function and stability of the 20S proteasome in the hyperthermophilic archaeon Pyrococcus furiosus.  |  Madding, LS., et al. 2007. J Bacteriol. 189: 583-90. PMID: 17114253

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-20S Proteasome β2 Antibody (MCP165) has 14 citations in a variety of scientific publications.

Primary antibodies like Anti-20S Proteasome β2 Antibody (MCP165) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

20S Proteasome β2 Antibody (MCP165) Specifications

Alternate Product Name: 20S Proteasome β2

Product Clone Name: MCP165

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: 20S Proteasome β2

Epitope Species: human

Product Reactivity/Detection: 20S Proteasome β2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 50 µg IgG₁ in 0.5 ml of PBS with < 0.1% sodium azide, 0.1% gelatin and 50% glycerol

Research Citations: 14 citations in various scientific publications

Available Conjugates/Forms for 20S Proteasome β2 Antibody (MCP165): N/A

The preferred 20S Proteasome β2 antibody is 20S Proteasome β2 Antibody (D-8): sc-515066.