20S Proteasome β1 Antibody (D-9): sc-374405

20S Proteasome β1 Antibody (D-9) is a mouse monoclonal IgG1 antibody that detects the 20S Proteasome beta 1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-20S Proteasome beta 1 antibody (D-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. 20S Proteasome beta 1 protein plays a crucial role in the cellular protein degradation pathway, which is essential for maintaining cellular homeostasis by removing damaged or misfolded proteins. This process is vital for regulating various cellular functions, including the cell cycle, apoptosis, and responses to oxidative stress. 20S Proteasome core, which is composed of multiple subunits, forms a cylindrical structure that houses the catalytic sites responsible for proteolysis. Understanding the function of 20S Proteasome beta 1 protein and its role in protein turnover is important for elucidating mechanisms of diseases where protein aggregation and degradation are disrupted, such as neurodegenerative disorders.

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20S Proteasome β1 Antibody (D-9) References:

1. Primary structural features of the 20S proteasome subunits of rice (Oryza sativa).  |  Sassa, H., et al. 2000. Gene. 250: 61-6. PMID: 10854779
2. Cloning and molecular characterization of a gene encoding a Cryptosporidium parvum putative 20S proteasome beta1-type subunit.  |  Chung, PA., et al. 2000. DNA Seq. 11: 309-14. PMID: 11092745
3. Catalytic site-specific inhibition of the 20S proteasome by 4-hydroxynonenal.  |  Ferrington, DA. and Kapphahn, RJ. 2004. FEBS Lett. 578: 217-23. PMID: 15589823
4. Optimization of subsite binding to the beta5 subunit of the human 20S proteasome using vinyl sulfones and 2-keto-1,3,4-oxadiazoles: syntheses and cellular properties of potent, selective proteasome inhibitors.  |  Rydzewski, RM., et al. 2006. J Med Chem. 49: 2953-68. PMID: 16686537
5. Inhibition on Proteasome β1 Subunit Might Contribute to the Anti-Cancer Effects of Fangchinoline in Human Prostate Cancer Cells.  |  Li, D., et al. 2015. PLoS One. 10: e0141681. PMID: 26512898
6. Phenoxypropanolamine derivatives as selective inhibitors of the 20S proteasome β1 and β5 subunits.  |  Hovhannisyan, AA., et al. 2017. Bioorg Med Chem Lett. 27: 5172-5178. PMID: 29113763
7. Ordered structure of the crystallized bovine 20S proteasome.  |  Morimoto, Y., et al. 1995. J Biochem. 117: 471-4. PMID: 7629009
8. Conformational constraints in protein degradation by the 20S proteasome.  |  Wenzel, T. and Baumeister, W. 1995. Nat Struct Biol. 2: 199-204. PMID: 7773788
9. Structure and structure formation of the 20S proteasome.  |  Schmidt, M., et al. 1997. Mol Biol Rep. 24: 103-12. PMID: 9228290
10. Human proteasome subunits from 2-dimensional gels identified by partial sequencing.  |  Kristensen, P., et al. 1994. Biochem Biophys Res Commun. 205: 1785-9. PMID: 7811265
11. Comprehensive mass spectrometric analysis of the 20S proteasome complex.  |  Huang, L. and Burlingame, AL. 2005. Methods Enzymol. 405: 187-236. PMID: 16413316
12. Role of the beta1 subunit in the function and stability of the 20S proteasome in the hyperthermophilic archaeon Pyrococcus furiosus.  |  Madding, LS., et al. 2007. J Bacteriol. 189: 583-90. PMID: 17114253

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-20S Proteasome β1 Antibody (D-9) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-20S Proteasome β1 Antibody (D-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

20S Proteasome β1 Antibody (D-9) Specifications

Alternate Product Name: PMSB1

Product Clone Name: D-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: 1-241

Epitope Target: 20S Proteasome β1

Epitope Species: human

Product Reactivity/Detection: 20S Proteasome β1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for 20S Proteasome β1 Antibody (D-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin