20S Proteasome α4 Antibody (H-4): sc-271297
- 20S Proteasome α4 Antibody (H-4) is a mouse monoclonal IgG1 κ, cited in 6 publications, provided at 200 µg/ml
- raised against amino acids 1-128 mapping at the N-terminus of 20S Proteasome α4 of human origin
- 20S Proteasome alpha 4 Antibody (H-4) is recommended for detection of 20S Proteasome α4 of mouse, rat, human and avian origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine, bovine and avian
- Anti-20S Proteasome alpha 4 Antibody (H-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for 20S Proteasome α4 Antibody (H-4) for WB applications. This reagent is now offered in a bundle with 20S Proteasome α4 Antibody (H-4) (see ordering information below).
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20S Proteasome α4 Antibody (H-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects 20S Proteasome α4 in mouse, rat, human, and avian samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-20S Proteasome α4 antibody (H-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. 20S proteasome plays a crucial role in cellular protein homeostasis by degrading unnecessary or damaged proteins, a process vital for maintaining cellular function and regulating various biological pathways. Located in the cytoplasm and nucleus, 20S proteasome is a core component of the larger 26S proteasome complex, which is essential for the selective degradation of ubiquitin-tagged proteins. The structure of 20S proteasome is composed of four stacked rings, with two outer rings made up of seven α subunits each, providing structural integrity and serving as docking sites for regulatory particles, while the inner rings consist of seven β subunits each, which are primarily responsible for the catalytic activity necessary for protein degradation. This unique architecture not only facilitates the efficient processing of substrates but also ensures that only properly tagged proteins gain access to the catalytic core, thereby preventing uncontrolled proteolysis and maintaining cellular health.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
20S Proteasome α4 Antibody (H-4) References:
- Primary structural features of the 20S proteasome subunits of rice (Oryza sativa). | Sassa, H., et al. 2000. Gene. 250: 61-6. PMID: 10854779
- Catalytic site-specific inhibition of the 20S proteasome by 4-hydroxynonenal. | Ferrington, DA. and Kapphahn, RJ. 2004. FEBS Lett. 578: 217-23. PMID: 15589823
- Comprehensive mass spectrometric analysis of the 20S proteasome complex. | Huang, L. and Burlingame, AL. 2005. Methods Enzymol. 405: 187-236. PMID: 16413316
- Optimization of subsite binding to the beta5 subunit of the human 20S proteasome using vinyl sulfones and 2-keto-1,3,4-oxadiazoles: syntheses and cellular properties of potent, selective proteasome inhibitors. | Rydzewski, RM., et al. 2006. J Med Chem. 49: 2953-68. PMID: 16686537
- Role of the beta1 subunit in the function and stability of the 20S proteasome in the hyperthermophilic archaeon Pyrococcus furiosus. | Madding, LS., et al. 2007. J Bacteriol. 189: 583-90. PMID: 17114253
- Ordered structure of the crystallized bovine 20S proteasome. | Morimoto, Y., et al. 1995. J Biochem. 117: 471-4. PMID: 7629009
- Conformational constraints in protein degradation by the 20S proteasome. | Wenzel, T. and Baumeister, W. 1995. Nat Struct Biol. 2: 199-204. PMID: 7773788
- Human proteasome subunits from 2-dimensional gels identified by partial sequencing. | Kristensen, P., et al. 1994. Biochem Biophys Res Commun. 205: 1785-9. PMID: 7811265
- Structure and structure formation of the 20S proteasome. | Schmidt, M., et al. 1997. Mol Biol Rep. 24: 103-12. PMID: 9228290
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
20S Proteasome α4 Antibody (H-4) | sc-271297 | 200 µg/ml | $316.00 | |||
20S Proteasome α4 Antibody (H-4): m-IgG Fc BP-HRP Bundle | sc-525798 | 200 µg Ab; 10 µg BP | $354.00 | |||
20S Proteasome α4 Antibody (H-4) AC | sc-271297 AC | 500 µg/ml, 25% agarose | $416.00 | |||
20S Proteasome α4 Antibody (H-4) HRP | sc-271297 HRP | 200 µg/ml | $316.00 | |||
20S Proteasome α4 Antibody (H-4) FITC | sc-271297 FITC | 200 µg/ml | $330.00 | |||
20S Proteasome α4 Antibody (H-4) PE | sc-271297 PE | 200 µg/ml | $343.00 | |||
20S Proteasome α4 Antibody (H-4) Alexa Fluor® 488 | sc-271297 AF488 | 200 µg/ml | $357.00 | |||
20S Proteasome α4 Antibody (H-4) Alexa Fluor® 546 | sc-271297 AF546 | 200 µg/ml | $357.00 | |||
20S Proteasome α4 Antibody (H-4) Alexa Fluor® 594 | sc-271297 AF594 | 200 µg/ml | $357.00 | |||
20S Proteasome α4 Antibody (H-4) Alexa Fluor® 647 | sc-271297 AF647 | 200 µg/ml | $357.00 | |||
20S Proteasome α4 Antibody (H-4) Alexa Fluor® 680 | sc-271297 AF680 | 200 µg/ml | $357.00 | |||
20S Proteasome α4 Antibody (H-4) Alexa Fluor® 790 | sc-271297 AF790 | 200 µg/ml | $357.00 |