2,2,7-trimethylguanosine Antibody (K121): sc-32724

2,2,7-trimethylguanosine Antibody (K121) is a mouse monoclonal IgG1 kappa light chain antibody that detects 2,2,7-trimethylguanosine in mouse, rat, and human samples through immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-2,2,7-trimethylguanosine antibody (K121) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. 2,2,7-trimethylguanosine plays a crucial role in gene expression regulation, particularly in pre-mRNA splicing, which is essential for accurate processing of precursor mRNA into mature mRNA. This nucleoside is found at the 5′ terminal cap site of small nuclear RNA (snRNA) components of U1, U2, U4/U6, and U5 nuclear small ribonucleoprotein particles (snRNPs), which are integral to the spliceosome complex that facilitates intron removal from precursor mRNA. 2,2,7-trimethylguanosine is vital for snRNP stability and functionality, and antibodies recognizing this cap structure can inhibit mRNA splicing, highlighting this modification′s importance in gene expression regulation. 2,2,7-trimethylguanosine monoclonal antibody (K121) ability to immunoprecipitate all major nuclear snRNPs makes this reagent invaluable for studying mRNA processing and molecular mechanisms of gene regulation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

2,2,7-trimethylguanosine Antibody (K121) References:

1. Preparation of N2, N2,7-trimethylguanosine affinity columns.  |  Espuny, R., et al. 1999. Nucleosides Nucleotides. 18: 125-36. PMID: 10048228
2. The fate of U1 snRNP during anti-Fas induced apoptosis: specific cleavage of the U1 snRNA molecule.  |  Degen, WG., et al. 2000. Cell Death Differ. 7: 70-9. PMID: 10713722
3. Purification, crystallization and preliminary crystallographic data of the m3G cap-binding domain of human snRNP import factor snurportin 1.  |  Strasser, A., et al. 2004. Acta Crystallogr D Biol Crystallogr. 60: 1628-31. PMID: 15333938
4. Genetic and biochemical analysis of yeast and human cap trimethylguanosine synthase: functional overlap of 2,2,7-trimethylguanosine caps, small nuclear ribonucleoprotein components, pre-mRNA splicing factors, and RNA decay pathways.  |  Hausmann, S., et al. 2008. J Biol Chem. 283: 31706-18. PMID: 18775984
5. Efficient solid-phase synthesis of oligodeoxynucleotides having a 5'-terminal 2,2,7-trimethylguanosine pyrophosphate linkage.  |  Ohkubo, A., et al. 2009. Bioorg Med Chem. 17: 4819-24. PMID: 19493681
6. How to find the optimal partner--studies of snurportin 1 interactions with U snRNA 5' TMG-cap analogues containing modified 2-amino group of 7-methylguanosine.  |  Piecyk, K., et al. 2015. Bioorg Med Chem. 23: 4660-4668. PMID: 26118337
7. Pre-mRNA splicing by complementation with purified human U1, U2, U4/U6 and U5 snRNPs.  |  Krainer, AR. 1988. Nucleic Acids Res. 16: 9415-29. PMID: 3141901
8. 5'-terminal caps of snRNAs are accessible for reaction with 2,2,7-trimethylguanosine-specific antibody in intact snRNPs.  |  Bringmann, P., et al. 1983. J Biol Chem. 258: 2745-7. PMID: 6186660
9. The 5' terminus of the RNA moiety of U1 small nuclear ribonucleoprotein particles is required for the splicing of messenger RNA precursors.  |  Krämer, A., et al. 1984. Cell. 38: 299-307. PMID: 6235919
10. Non-radioactive detection of m3G capped RNAs by immunoblotting.  |  Stamm, S., et al. 1994. Nucleic Acids Res. 22: 252-3. PMID: 8121812
11. A 7-methylguanosine cap commits U3 and U8 small nuclear RNAs to the nucleolar localization pathway.  |  Jacobson, MR. and Pederson, T. 1998. Nucleic Acids Res. 26: 756-60. PMID: 9443967
12. Solid phase synthesis of U1 RNA oligomers containing a TMG cap structure.  |  Kadokura, M., et al. 1997. Nucleic Acids Symp Ser. 77-8. PMID: 9586007

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-2,2,7-trimethylguanosine Antibody (K121) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-2,2,7-trimethylguanosine Antibody (K121) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

2,2,7-trimethylguanosine Antibody (K121) Specifications

Alternate Product Name: 2,2,7-trimethylguanosine

Product Clone Name: K121

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: 2,2,7-trimethylguanosine

Epitope Species: keyhole limpet

Product Reactivity/Detection: 5` terminal 2,2,7-trimethylguanosine of all major snRNAs

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for 2,2,7-trimethylguanosine Antibody (K121): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin