15-LO2 Double Nickase Plasmid (h): sc-404699-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- 15-LO2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- 15-LO2 Double Nickase Plasmid (h) and 15-LO2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ALOX15B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: 15-LO2 Antibody (F-10): sc-376795
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
15-LO2 Double Nickase Plasmid (h) | sc-404699-NIC | 20 µg | $410.00 | |||
15-LO2 Double Nickase Plasmid (h2) | sc-404699-NIC-2 | 20 µg | $410.00 |
Human ALOX15B encodes 15-lipoxygenase-2 (15-LO2), a non-heme iron dioxygenase that oxygenates polyunsaturated fatty acids to generate bioactive lipid mediators such as 15-HETE from arachidonic acid. Through regulation of eicosanoid and specialized pro-resolving mediator networks, 15-LO2 influences inflammatory signaling, epithelial differentiation, redox homeostasis, and membrane lipid remodeling. ALOX15B activity intersects with arachidonic acid metabolism, PPAR signaling, and cytokine-driven pathways that shape tissue responses to injury and immune cues. Dysregulated expression or altered lipid product profiles have been associated with inflammation-linked pathologies and cancer-related changes in epithelial cell behavior, making it a useful target for mechanistic studies of lipid signaling.
15-LO2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ALOX15B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ALOX15B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ALOX15B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ALOX15B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.