Date published: 2026-4-24

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14-3-3 ζ (h): 293T Lysate: sc-111414

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Datasheets
  • human 14-3-3 ζ transfected 293T lysate
  • 100 µg protein in 200 µl SDS-PAGE buffer; suitable as Western Blotting control for 14-3-3 ζ antibodies
  • Suitable control antibody: pan 14-3-3 Antibody (F-1): sc-166061 is recommended as control antibody for monitoring of 14-3-3 ζ expression knockdown by Western blotting or immunofluorescence
  • should be stored at -20° C and repeated freezing and thawing should be minimized
  • sample vial should be boiled once prior to use
  • non-transfected control 293T lysate (sc-117752)
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    The 14-3-3 ζ (h): 293T Lysate is enriched with the 14-3-3 ζ protein, an important regulatory molecule involved in numerous cellular processes such as signal transduction, apoptosis, and cell cycle control. The 14-3-3 proteins are known for their role in binding to phosphorylated serine and threonine residues, influencing the activity, localization, and stability of their target proteins. In research, the 14-3-3 ζ (h): 293T Lysate is utilized to investigate the molecular interactions and regulatory mechanisms mediated by the 14-3-3 ζ isoform. It is particularly valuable in co-immunoprecipitation assays to identify and study 14-3-3 ζ binding partners and their functional implications. Additionally, this lysate is employed in Western blotting to confirm the presence and quantify the expression levels of 14-3-3 ζ and its associated proteins under various experimental conditions. Researchers also use this lysate in electrophoretic mobility shift assays (EMSA) to analyze DNA-binding protein activities influenced by 14-3-3 ζ interactions. The use of 14-3-3 ζ (h): 293T Lysate allows for the detailed exploration of signal transduction pathways and protein networks involving 14-3-3 ζ, providing insights into its role in cellular regulation and dynamics. The origin and quality of the 293T cell line are meticulously controlled, ensuring reliable and reproducible results in experimental setups focused on fundamental biological processes.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    14-3-3 ζ (h): 293T Lysate

    sc-111414
    100 µg/200 µl
    $236.00