Date published: 2026-6-26
Visite Santa Cruz Saúde Animal

1-800-457-3801

SCBT Portrait Logo
Seach Input
Entre em Contato Conosco
Subscrever / Inscrever-se Conta
Rápido Pedido de compra
Corrinho de compra (0)
The Double Nickase Plasmid features a U6 promoter for sgRNA expression, a 20 nt targeting sequence, and a gRNA scaffold to guide Cas9n. It includes a CBh promoter for Cas9n (D10A) and puromycin resistance, GFP for transfection verification, and nuclear localization signals (NLS). The 2A peptide allows co-expression of Cas9n and Puro from a single promoter, enabling precise genome editing with reduced off-target effects.
The Double Nickase Plasmid features a U6 promoter for sgRNA expression, a 20 nt targeting sequence, and a gRNA scaffold to guide Cas9n. It includes a CBh promoter for Cas9n (D10A) and puromycin resistance, GFP for transfection verification, and nuclear localization signals (NLS). The 2A peptide allows co-expression of Cas9n and Puro from a single promoter, enabling precise genome editing with reduced off-target effects.
The Double Nickase Plasmid features a U6 promoter for sgRNA expression, a 20 nt targeting sequence, and a gRNA scaffold to guide Cas9n. It includes a CBh promoter for Cas9n (D10A) and puromycin resistance, GFP for transfection verification, and nuclear localization signals (NLS). The 2A peptide allows co-expression of Cas9n and Puro from a single promoter, enabling precise genome editing with reduced off-target effects.
The Double Nickase Plasmid features a U6 promoter for sgRNA expression, a 20 nt targeting sequence, and a gRNA scaffold to guide Cas9n. It includes a CBh promoter for Cas9n (D10A) and puromycin resistance, GFP for transfection verification, and nuclear localization signals (NLS). The 2A peptide allows co-expression of Cas9n and Puro from a single promoter, enabling precise genome editing with reduced off-target effects.
Cas9n Nickase gRNA Plasmid Targeting: Dual gRNA plasmids create single-strand nicks at precise DNA sequences for efficient genome editing using Cas9n Nickase.
Cas9n Nickase gRNA Plasmid Targeting: Dual gRNA plasmids create single-strand nicks at precise DNA sequences for efficient genome editing using Cas9n Nickase.
This image illustrates the Cas9n Nickase mechanism used for precise genome editing. Two plasmids (Plasmid 1 and Plasmid 2) are shown, each containing a targeted DNA sequence. The system utilizes single-guide RNAs (sgRNA) to direct Cas9n Nickase to specific genomic locations, represented by the blue and pink DNA strands. The sgRNA scaffold aids in guiding Cas9n to the 20 nucleotide (nt) target sequence on the DNA. Cas9n makes single-strand cuts at NCC and NGG sites, enabling precise gene modifications without creating double-strand breaks.
This image illustrates the Cas9n Nickase mechanism used for precise genome editing. Two plasmids (Plasmid 1 and Plasmid 2) are shown, each containing a targeted DNA sequence. The system utilizes single-guide RNAs (sgRNA) to direct Cas9n Nickase to specific genomic locations, represented by the blue and pink DNA strands. The sgRNA scaffold aids in guiding Cas9n to the 20 nucleotide (nt) target sequence on the DNA. Cas9n makes single-strand cuts at NCC and NGG sites, enabling precise gene modifications without creating double-strand breaks.
The Double Nickase Plasmid features a U6 promoter for sgRNA expression, a 20 nt targeting sequence, and a gRNA scaffold to guide Cas9n. It includes a CBh promoter for Cas9n (D10A) and puromycin resistance, GFP for transfection verification, and nuclear localization signals (NLS). The 2A peptide allows co-expression of Cas9n and Puro from a single promoter, enabling precise genome editing with reduced off-target effects.

γ Enolase Plasmídeo duplo de Nickase (h): sc-400763-NIC

0.0(0)
Escrever uma avaliaçãoFazer uma pergunta

Fichas de dados
  • alvos específicos: human
  • 20 µg de plasmídeo de DNA pronto para transfecção; Suficiente para até 20 transfecções
  • γ EnolaseO plasmídeo de dupla Nickase (h) consiste num par de plasmídeos cada um contem D10A com mutação na nuclease Cas9 nuclease e um alvo especifico de RNA guia com 20 na (gRNA), criado para nocautear a expressão genética com maior especificidade do que o seu correspondente CRISPR/Cas9 KO
  • Sequencias pareadas de gRNA são de aproximadamente 20 bp para permitir que os cortes duplos no DNA genômico mediados pela Casp ocorram com especificidade , o que se assemelha ao corte pela DSB
  • Cada plasmídeo pertencente ao par de plasmídeos contem um gene de resistência a puromicina para seleção; o outro plasmídeo do par contem um marcador de GFP para a visualização e confirmação da transfecção
  • O Plasmídeo Double Nickase γ Enolase (h) e o Plasmídeo Double Nickase γ Enolase (h2) codificam designs distintos de pares de gRNA direcionados para ENO2. Um ou ambos os desenhos podem estar disponíveis
  • Após a transfecção, a eficácia do processo de nocaute genético por ser testada WB, IF ou IHC usando o anticorpo:γ Enolase Anticorpo (D-7): sc-376375
    Gene Editing Promo Banner

    Informacoes sobre ordens

    Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

    γ Enolase Plasmídeo duplo de Nickase (h)

    sc-400763-NIC
    20 µg
    		$410.00

    γ Enolase Plasmídeo duplo de Nickase (h2)

    sc-400763-NIC-2
    20 µg
    		$410.00
    1-800-457-3801
    Visite Santa Cruz Animal Health|Visite San Juan Ranch
    A Santa Cruz Biotecnologia, Inc. é a líder mundial no desenvolvimento de produtos para o mercado de pesquisa biomédica. Ligue para nós ligação gratuita 1-800-457-3801..Direito de Reprodução© 2007-2026 Santa Cruz Biotechnology, Inc. Todos os direitos reservados. “Santa Cruz Biotechnology”, Santa Cruz Biotechnology, Inc. logo, “Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch logo, “UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, “EZ Touch” são marcas registradas da Santa Cruz Biotechnology, Inc. Todas as marcas registradas são propriedade dos seus respectivos donos.