γ-catenin CRISPR Activation Plasmid (h): sc-401640-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- γ-catenin CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- γ-catenin CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by γ-catenin CRISPR Activation Plasmid (h) and γ-catenin CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the JUP transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: γ-catenin Antibody (A-6): sc-514115
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
γ-catenin CRISPR Activation Plasmid (h) | sc-401640-ACT | 20 µg | $397.00 |
JUP encodes γ-catenin (plakoglobin), an armadillo repeat protein that functions as a core component of desmosomes and adherens junctions, linking cadherin complexes to intermediate filaments and actin-associated networks to support epithelial and cardiac tissue integrity. Through interactions with desmogleins, desmocollins, plakophilins, and desmoplakin, γ-catenin coordinates junctional assembly, mechanotransduction, and contact-dependent regulation of cell migration and differentiation. γ-catenin also participates in crosstalk with Wnt/β-catenin signaling by modulating transcriptional programs and junction-to-nucleus signaling dynamics. Dysregulated JUP expression or junctional localization is associated with altered adhesion, invasive behavior, and cardiomyocyte structural defects, making it relevant for studies of barrier function, tissue remodeling, and disease-associated signaling.
γ-catenin CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous JUP expression without altering the underlying DNA sequence.
γ-catenin CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the JUP locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the JUP transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous γ-catenin expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native JUP locus and enabling the study of γ-catenin-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of γ-catenin pathway restoration in tumor cells with silenced or reduced JUP expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.