Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
γB-crystallin CRISPR Activation Plasmid (h): sc-402939-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- γB-crystallin CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- γB-crystallin CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by γB-crystallin CRISPR Activation Plasmid (h) and γB-crystallin CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the CRYGB transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: γB-crystallin Antibody (D-5): sc-377056
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
γB-crystallin CRISPR Activation Plasmid (h) | sc-402939-ACT | 20 µg | $397.00 | |||
γB-crystallin CRISPR Activation Plasmid (h2) | sc-402939-ACT-2 | 20 µg | $397.00 |
CRYGB encodes human γB-crystallin, a highly abundant, long-lived structural protein of the eye lens that contributes to refractive index and optical transparency through dense yet soluble protein packing. As a member of the β/γ-crystallin superfamily, γB-crystallin supports lens fiber cell homeostasis by maintaining protein stability and resisting aggregation under oxidative and osmotic stress conditions. Disruption of crystallin proteostasis is linked to light-scattering aggregates and lens opacification, and CRYGB variants have been associated with inherited cataract phenotypes. Consequently, CRYGB is widely used to study lens development, protein folding/aggregation, and stress-responsive pathways that influence transparency.
γB-crystallin CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous CRYGB expression without altering the underlying DNA sequence.
γB-crystallin CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the CRYGB locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the CRYGB transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous γB-crystallin expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native CRYGB locus and enabling the study of γB-crystallin-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of γB-crystallin pathway restoration in tumor cells with silenced or reduced CRYGB expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.