δ-casein CRISPR/Cas9 KO Plasmid (m): sc-419849
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- δ-casein CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the δ-casein genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
δ-casein CRISPR/Cas9 KO Plasmid (m) | sc-419849 | 20 µg | $397.00 |
Overview
Csn1s2b encodes δ-casein, a secreted milk protein expressed in the mammary gland that contributes to casein micelle structure and calcium/phosphate binding during lactation. As part of the casein gene cluster, its transcription is tightly regulated across pregnancy and lactation by prolactin/JAK2–STAT5 signaling and other hormone-responsive pathways that coordinate milk protein synthesis and secretion. Altered casein expression patterns are used as readouts of mammary epithelial differentiation and lactogenic competence, and casein family genes are frequently monitored in studies of mammary gland development and stress responses. In mouse models, perturbation of casein components can inform mechanisms underlying milk composition, neonatal nutrition, and lactation-related phenotypes.
δ-casein CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Csn1s2b gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Csn1s2b together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Csn1s2b open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish δ-casein protein expression.
This CRISPR knockout system enables efficient generation of Csn1s2b-deficient cell models for investigation of δ-casein signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Csn1s2b exon(s) critical for δ-casein function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Csn1s2b genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by δ-casein CRISPR/Cas9 KO Plasmid (m) and δ-casein CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Csn1s2b locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by δ-casein HDR Plasmid (m) and δ-casein HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Csn1s2b homology arms to support homology-directed repair at defined Csn1s2b target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.