β8 Tubulin CRISPR/Cas9 KO Plasmid (h): sc-401438
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- β8 Tubulin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the β8 Tubulin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
β8 Tubulin CRISPR/Cas9 KO Plasmid (h) | sc-401438 | 20 µg | $397.00 |
Overview
TUBB8 encodes β8 tubulin, a β-tubulin isotype that polymerizes with α-tubulin to form microtubules, supporting cytoskeletal architecture, intracellular transport, and spindle dynamics during cell division. Microtubule remodeling underlies processes such as mitosis, organelle positioning, and cell polarity through coordinated regulation by microtubule-associated proteins and post-translational tubulin modifications. Altered tubulin isotype composition can influence microtubule stability and sensitivity to microtubule-targeting stress, linking tubulin biology to defects in chromosome segregation and cellular homeostasis. TUBB8 has been studied in the context of reproductive and developmental phenotypes where microtubule organization is critical, providing a model for dissecting isotype-specific contributions to microtubule function.
β8 Tubulin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TUBB8 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TUBB8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TUBB8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish β8 Tubulin protein expression.
This CRISPR knockout system enables efficient generation of TUBB8-deficient cell models for investigation of β8 Tubulin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TUBB8 exon(s) critical for β8 Tubulin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TUBB8 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by β8 Tubulin CRISPR/Cas9 KO Plasmid (h) and β8 Tubulin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TUBB8 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by β8 Tubulin HDR Plasmid (h) and β8 Tubulin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TUBB8 homology arms to support homology-directed repair at defined TUBB8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.