β4 Tubulin CRISPR/Cas9 KO Plasmid (h): sc-400058
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- β4 Tubulin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the β4 Tubulin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
β4 Tubulin CRISPR/Cas9 KO Plasmid (h) | sc-400058 | 20 µg | $397.00 |
Overview
TUBB4A encodes β4 tubulin, a neuron-enriched β-tubulin isotype that polymerizes with α-tubulin to form microtubules, supporting mitotic spindle architecture, intracellular transport, and neurite outgrowth. Dynamic microtubule turnover underpins processes including axonal trafficking and cytoskeletal remodeling, integrating with pathways that coordinate cell cycle progression and neuronal differentiation. Altered TUBB4A function perturbs microtubule assembly and stability, linking this gene to neurodevelopmental and neurodegenerative phenotypes in genetic studies. As a core cytoskeletal component, β4 tubulin provides a mechanistic entry point for investigating microtubule-dependent signaling and vulnerability to cytoskeletal stress in human cell models.
β4 Tubulin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TUBB4A gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TUBB4A together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TUBB4A open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish β4 Tubulin protein expression.
This CRISPR knockout system enables efficient generation of TUBB4A-deficient cell models for investigation of β4 Tubulin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TUBB4A exon(s) critical for β4 Tubulin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TUBB4A genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by β4 Tubulin CRISPR/Cas9 KO Plasmid (h) and β4 Tubulin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TUBB4A locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by β4 Tubulin HDR Plasmid (h) and β4 Tubulin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TUBB4A homology arms to support homology-directed repair at defined TUBB4A target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.