Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
β2C Tubulin CRISPR Activation Plasmid (h): sc-400056-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- β2C Tubulin CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- β2C Tubulin CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by β2C Tubulin CRISPR Activation Plasmid (h) and β2C Tubulin CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the TUBB4B transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: β2C Tubulin Antibody (1A9): sc-134230
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
β2C Tubulin CRISPR Activation Plasmid (h) | sc-400056-ACT | 20 µg | $397.00 | |||
β2C Tubulin CRISPR Activation Plasmid (h2) | sc-400056-ACT-2 | 20 µg | $397.00 |
TUBB4B encodes β2C Tubulin, a core β-tubulin isotype that polymerizes with α-tubulins to form microtubules, supporting cytoskeletal architecture, intracellular transport, and mitotic spindle assembly. Microtubule dynamics driven by tubulin heterodimers regulate cell cycle progression, vesicle trafficking, and neuronal process organization through pathways involving motor proteins such as kinesins and dynein. Altered tubulin isotype expression and microtubule remodeling are frequently studied in contexts of chromosomal instability, impaired cell polarity, and stress responses that accompany oncogenic transformation. Because microtubules are central to division and migration, TUBB4B is broadly relevant for research into cytoskeletal regulation, differentiation, and microenvironment-driven phenotypes.
β2C Tubulin CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous TUBB4B expression without altering the underlying DNA sequence.
β2C Tubulin CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the TUBB4B locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the TUBB4B transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous β2C Tubulin expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native TUBB4B locus and enabling the study of β2C Tubulin-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of β2C Tubulin pathway restoration in tumor cells with silenced or reduced TUBB4B expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.