Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
β2B Tubulin CRISPR/Cas9 KO Plasmid (h): sc-400059
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- β2B Tubulin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the β2B Tubulin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: β2B Tubulin Antibody (AT5B3): sc-517430
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
β2B Tubulin CRISPR/Cas9 KO Plasmid (h) | sc-400059 | 20 µg | $397.00 |
Overview
TUBB2B encodes β2B tubulin, a neuron-enriched β-tubulin isotype that heterodimerizes with α-tubulin to assemble microtubules and support cytoskeletal integrity. Microtubule dynamics driven by tubulin polymers regulate mitotic spindle organization, intracellular trafficking, neurite outgrowth, and neuronal migration, linking TUBB2B to fundamental processes such as cell division and neurodevelopment. As a component of the microtubule cytoskeleton, β2B tubulin interfaces with pathways controlling axonal transport and cortical layering through interactions with microtubule-associated proteins and motor complexes. Pathogenic variation in TUBB2B has been associated with tubulinopathies and malformations of cortical development, providing a genetic entry point for studying cytoskeletal mechanisms underlying neurodevelopmental phenotypes.
β2B Tubulin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TUBB2B gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TUBB2B together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TUBB2B open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish β2B Tubulin protein expression.
This CRISPR knockout system enables efficient generation of TUBB2B-deficient cell models for investigation of β2B Tubulin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TUBB2B exon(s) critical for β2B Tubulin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TUBB2B genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by β2B Tubulin CRISPR/Cas9 KO Plasmid (h) and β2B Tubulin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TUBB2B locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by β2B Tubulin HDR Plasmid (h) and β2B Tubulin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TUBB2B homology arms to support homology-directed repair at defined TUBB2B target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.