Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
β2A Tubulin CRISPR Activation Plasmid (h): sc-400054-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- β2A Tubulin CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- β2A Tubulin CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by β2A Tubulin CRISPR Activation Plasmid (h) and β2A Tubulin CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the TUBB2A transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: β2A Tubulin Antibody (2-RY22): sc-134229
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
β2A Tubulin CRISPR Activation Plasmid (h) | sc-400054-ACT | 20 µg | $397.00 | |||
β2A Tubulin CRISPR Activation Plasmid (h2) | sc-400054-ACT-2 | 20 µg | $397.00 |
TUBB2A encodes β2A Tubulin, a core α/β-tubulin heterodimer component that polymerizes into microtubules to support cytoskeletal architecture, intracellular trafficking, and mitotic spindle dynamics. By regulating microtubule growth and stability, β2A Tubulin contributes to processes including neuronal migration, axon guidance, and cell-cycle progression through microtubule-dependent pathways. Altered tubulin isotype expression or microtubule network dysfunction is frequently investigated in contexts such as neurodevelopmental phenotypes, chromosomal instability, and aberrant cell division. As a cytoskeletal hub, TUBB2A provides a mechanistic entry point for studying microtubule-associated proteins, motor transport, and mitosis-linked signaling.
β2A Tubulin CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous TUBB2A expression without altering the underlying DNA sequence.
β2A Tubulin CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the TUBB2A locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the TUBB2A transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous β2A Tubulin expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native TUBB2A locus and enabling the study of β2A Tubulin-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of β2A Tubulin pathway restoration in tumor cells with silenced or reduced TUBB2A expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.