β-1,4-Gal-T4 CRISPR/Cas9 KO Plasmid (h): sc-407251

Overview

B4GALT4 encodes β-1,4-Gal-T4, a Golgi-resident β-1,4-galactosyltransferase that transfers galactose to N-acetylglucosamine to build type II lactosamine units on N- and O-linked glycans and glycolipids. Through its contribution to glycosylation, β-1,4-Gal-T4 influences protein folding, trafficking, receptor residence time, and cell–cell or cell–matrix interactions mediated by glycan-binding proteins. Altered galactosylation and lactosamine extension can reshape signaling networks and immune recognition, linking B4GALT4-dependent glycan remodeling to disease-relevant processes such as inflammation and tumor cell behavior. As part of the broader glycosyltransferase network, B4GALT4 helps define cell-surface glycome composition that impacts adhesion, migration, and differentiation programs.

β-1,4-Gal-T4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the B4GALT4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the B4GALT4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the B4GALT4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish β-1,4-Gal-T4 protein expression.

This CRISPR knockout system enables efficient generation of B4GALT4-deficient cell models for investigation of β-1,4-Gal-T4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting B4GALT4 exon(s) critical for β-1,4-Gal-T4 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple B4GALT4 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by β-1,4-Gal-T4 CRISPR/Cas9 KO Plasmid (h) and β-1,4-Gal-T4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the B4GALT4 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by β-1,4-Gal-T4 HDR Plasmid (h) and β-1,4-Gal-T4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by B4GALT4 homology arms to support homology-directed repair at defined B4GALT4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.