Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
α1D-AR CRISPR/Cas9 KO Plasmid (m): sc-419022
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- α1D-AR CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the α1D-AR genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: α1D-AR Antibody (F-10): sc-390884
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
α1D-AR CRISPR/Cas9 KO Plasmid (m) | sc-419022 | 20 µg | $397.00 |
Overview
Adra1d encodes the mouse α1D-adrenergic receptor (α1D-AR), a G protein-coupled receptor that primarily couples to Gq/11 to stimulate phospholipase C signaling, inositol phosphate production, intracellular Ca2+ mobilization, and PKC activation. Through these pathways, α1D-AR influences smooth muscle contraction, vascular and urinary tract tone, and broader autonomic regulation, with downstream effects on MAPK/ERK signaling and transcriptional programs. Adra1d expression and signaling dynamics are frequently examined in the context of tissue-specific adrenergic responsiveness and receptor subtype contributions to physiological homeostasis. Dysregulated adrenergic GPCR signaling has been linked to cardiometabolic and neurobehavioral phenotypes in preclinical models, making Adra1d a relevant target for mechanistic studies of stress-responsive pathways.
α1D-AR CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Adra1d gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Adra1d together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Adra1d open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish α1D-AR protein expression.
This CRISPR knockout system enables efficient generation of Adra1d-deficient cell models for investigation of α1D-AR signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Adra1d exon(s) critical for α1D-AR function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Adra1d genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by α1D-AR CRISPR/Cas9 KO Plasmid (m) and α1D-AR CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Adra1d locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by α1D-AR HDR Plasmid (m) and α1D-AR HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Adra1d homology arms to support homology-directed repair at defined Adra1d target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.