α Enolase Double Nickase Plasmid (h): sc-400633-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- α Enolase Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- α Enolase Double Nickase Plasmid (h) and α Enolase Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ENO1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: α Enolase Antibody (9): sc-101513
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
α Enolase Double Nickase Plasmid (h) | sc-400633-NIC | 20 µg | $410.00 | |||
α Enolase Double Nickase Plasmid (h2) | sc-400633-NIC-2 | 20 µg | $410.00 |
ENO1 encodes human α enolase, a bifunctional enzyme that catalyzes the 2-phosphoglycerate to phosphoenolpyruvate step in glycolysis and contributes broadly to cellular energy metabolism. Beyond its metabolic role, α enolase has been implicated in plasminogen binding at the cell surface and in stress-responsive signaling programs that influence proliferation, migration, and survival. ENO1 activity interfaces with glycolytic rewiring, hypoxia-adaptation pathways, and other metabolic processes frequently examined in cancer biology and inflammatory settings. Dysregulated ENO1 expression or localization is associated with altered metabolic phenotypes and has been studied in contexts including tumor progression, autoimmunity, and neurodegenerative disease models.
α Enolase Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ENO1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ENO1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ENO1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ENO1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.