α Enolase CRISPR/Cas9 KO Plasmid (m): sc-420178
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- α Enolase CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the α Enolase genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: α Enolase Antibody (9): sc-101513
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
α Enolase CRISPR/Cas9 KO Plasmid (m) | sc-420178 | 20 µg | $397.00 |
Overview
Eno1 encodes α enolase, a conserved metalloenzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis, supporting ATP generation and carbon flux under diverse nutrient conditions. Beyond its canonical metabolic role, ENO1 contributes to broader metabolic reprogramming and can influence redox balance, hypoxia adaptation, and cellular stress responses through its impact on glycolytic throughput. Altered ENO1 expression and activity have been associated with proliferative and inflammatory phenotypes, and it is frequently studied in contexts where energy metabolism intersects with oncogenic signaling and immune cell function. In mouse systems, Eno1 serves as a tractable node for dissecting glycolysis-linked mechanisms that shape cell growth, migration, and responses to microenvironmental cues.
α Enolase CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Eno1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Eno1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Eno1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish α Enolase protein expression.
This CRISPR knockout system enables efficient generation of Eno1-deficient cell models for investigation of α Enolase signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Eno1 exon(s) critical for α Enolase function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Eno1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by α Enolase CRISPR/Cas9 KO Plasmid (m) and α Enolase CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Eno1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by α Enolase HDR Plasmid (m) and α Enolase HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Eno1 homology arms to support homology-directed repair at defined Eno1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.