Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
α-2 antiplasmin CRISPR/Cas9 KO Plasmid (h): sc-403792
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- α-2 antiplasmin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the α-2 antiplasmin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: α-2 antiplasmin Antibody (C-7): sc-515771
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
α-2 antiplasmin CRISPR/Cas9 KO Plasmid (h) | sc-403792 | 20 µg | $397.00 |
Overview
SERPINF2 encodes human α-2 antiplasmin, a circulating serine protease inhibitor that is the principal physiological inhibitor of plasmin and a key regulator of fibrinolysis. By forming stable complexes with plasmin and being crosslinked into fibrin by factor XIIIa, α-2 antiplasmin limits fibrin clot breakdown and helps balance coagulation and extracellular proteolysis. This activity connects SERPINF2 to the plasminogen activation system, fibrin remodeling, and downstream effects on matrix turnover and vascular homeostasis. Altered SERPINF2 function or expression has been associated with dysregulated fibrinolytic capacity and is studied in the context of bleeding and thrombosis phenotypes, as well as inflammatory and tissue remodeling settings where plasmin activity is consequential.
α-2 antiplasmin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SERPINF2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SERPINF2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SERPINF2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish α-2 antiplasmin protein expression.
This CRISPR knockout system enables efficient generation of SERPINF2-deficient cell models for investigation of α-2 antiplasmin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SERPINF2 exon(s) critical for α-2 antiplasmin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SERPINF2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by α-2 antiplasmin CRISPR/Cas9 KO Plasmid (h) and α-2 antiplasmin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SERPINF2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by α-2 antiplasmin HDR Plasmid (h) and α-2 antiplasmin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SERPINF2 homology arms to support homology-directed repair at defined SERPINF2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.