α1a Tubulin Double Nickase Plasmid (h): sc-400022-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- α1a Tubulin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- α1a Tubulin Double Nickase Plasmid (h) and α1a Tubulin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TUBA1A. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: α1a Tubulin Antibody (7-RY28): sc-134237
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
α1a Tubulin Double Nickase Plasmid (h) | sc-400022-NIC | 20 µg | $410.00 | |||
α1a Tubulin Double Nickase Plasmid (h2) | sc-400022-NIC-2 | 20 µg | $410.00 |
TUBA1A encodes α1a tubulin, a core component of microtubules that polymerize into dynamic filaments required for mitotic spindle formation, intracellular trafficking, and maintenance of cell polarity. α1a tubulin supports cytoskeletal remodeling and microtubule-dependent processes across the cell cycle, enabling proper chromosome segregation and neuronal morphogenesis. Altered TUBA1A function is linked to disrupted microtubule dynamics and abnormal cortical development, making it relevant to studies of neurodevelopmental disorders and cytoskeletal dysregulation. As a major α-tubulin isoform in humans, it is frequently used to interrogate microtubule assembly, stability, and interactions with microtubule-associated proteins and motor complexes.
α1a Tubulin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TUBA1A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TUBA1A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TUBA1A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TUBA1A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.