Date published: 2025-9-8
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Vitamin D Receptor/VDR Antibody (D-6) - Western Blotting - Image 362199
VDR 항체 (D-6): sc-13133. HeLa (A), MCF7 (B) 및 C3H/10T1/2 (C) 전체 세포 용해물에서 VDR 발현의 웨스턴 블롯 분석.
VDR 항체 (D-6): sc-13133. HeLa (A), MCF7 (B) 및 C3H/10T1/2 (C) 전체 세포 용해물에서 VDR 발현의 웨스턴 블롯 분석.

Vitamin D Receptor/VDR 항체 (D-6): sc-13133

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데이터 시트
  • Vitamin D Receptor/VDR 항체 D-6 는 마우스 monoclonal IgG2a κ Vitamin D Receptor/VDR 항체, 437간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human origin의 VDR에서 내부에 위치한 344-424 아미노산을 항원으로 사용하였습니다.
  • Vitamin D Receptor/VDR 항체 (D-6)는 WB, IP, IF and IHC(P)으로 mouse, rat and human유래의 VDR 를 감지하는 데에 추천한다.
  • 항-Vitamin D Receptor/VDR 항체(D-6)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • WB, IHC(P) 와 ELISA에 사용가능한 biotin결합제품 이 있습니다.
  • Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-13133 X, 200 µg/0.1 ml)이 있습니다.
  • Vitamin D Receptor/VDR (D-6): sc-13133의 무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP 와 m-IgG2a BP-HRP 는 Vitamin D Receptor/VDR 항체(D-6) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은Vitamin D Receptor/VDR 항체(D-6) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다).

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VDR 항체(D-6)는 WB, IP, IF 및 IHC(P)에 의해 마우스, 랫트 및 인간 기원의 VDR 단백질을 검출하는 IgG2a κ 마우스 단일 클론 VDR 항체(Vitamin D 수용체 항체, 또는 NR1I1 항체로도 지정됨)이다. VDR 항체(D-6)는 비 conjug 결합 항-VDR 항체 형태뿐만 아니라 아가로오스, HRP, PE, FITC 및 다중 Alexa Fluor® 접합체를 포함한 다중 접합 형태의 항-VDR 항체로서 이용 가능하다. 비타민 D의 활성 대사산물은 발달적으로 특이적인 방식으로 매우 다양한 유전자의 발현을 조절한다. 이 시코스테로이드 호르몬은 증식, 분화 및 칼슘 항상성과 같은 다양한 반응 배열에 관여하는 유전자의 발현을 상향 또는 하향 조절할 수 있다. 1,25-(OH)2-비타민 D3는 호르몬 활성화 핵 수용체 상과의 구성체인 비타민 D 수용체(VDR)와의 상호작용을 통해 그 효과를 발휘한다. 리간드 결합 상태에서 VDR은 9-cis 레티노익산 수용체인 RXR과 이종이량체를 형성하고 호르몬 반응 요소, 또는 HRE로 알려진 특정 DNA 서열을 결합함으로써 유전자 발현에 영향을 미친다. 상기 언급된 세포 반응을 조절하는 것 외에도 1,25-(OH)2-비타민 D3는 골육종, 흑색종, 대장암 및 유방암 세포에서 항증식 특성을 나타낸다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

Vitamin D Receptor/VDR 항체 (D-6) 참고문헌:

  1. 비타민 D 매개 유전자 발현.  |  Lowe, KE., et al. 1992. Crit Rev Eukaryot Gene Expr. 2: 65-109. PMID: 1543898
  2. 비타민 D와 전립선암.  |  Feldman, D., et al. 1995. Adv Exp Med Biol. 375: 53-63. PMID: 7645428
  3. 1,25-디하이드록시비타민 D3의 게놈 작용.  |  Whitfield, GK., et al. 1995. J Nutr. 125: 1690S-1694S. PMID: 7782928
  4. 유방암 세포의 비타민 D 수용체.  |  Buras, RR., et al. 1994. Breast Cancer Res Treat. 31: 191-202. PMID: 7881099
  5. 골다공증 관리에서 비타민 D, 그 대사체 및 유사체의 역할.  |  Bikle, DD. 1994. Rheum Dis Clin North Am. 20: 759-75. PMID: 7984788
  6. 비타민 D에 의한 분화 신호 경로: 증식성 및 종양성 질환 치료에 대한 시사점.  |  Studzinski, GP., et al. 1993. Crit Rev Eukaryot Gene Expr. 3: 279-312. PMID: 8286848

주문정보

제품명카탈로그 번호 단위가격수량관심품목

Vitamin D Receptor/VDR 항체 (D-6)

sc-13133
200 µg/ml
$316.00

Vitamin D Receptor/VDR (D-6): m-IgG Fc BP-HRP 번들

sc-525466
200 µg Ab; 10 µg BP
$354.00

Vitamin D Receptor/VDR (D-6): m-IgG2a BP-HRP 번들

sc-546272
200 µg Ab; 10 µg BP
$354.00

Vitamin D Receptor/VDR 항체 (D-6) X

sc-13133 X
200 µg/0.1 ml
$316.00

Vitamin D Receptor/VDR 항체 (D-6) AC

sc-13133 AC
500 µg/ml, 25% agarose
$416.00

Vitamin D Receptor/VDR 항체 (D-6) HRP

sc-13133 HRP
200 µg/ml
$316.00

Vitamin D Receptor/VDR 항체 (D-6) FITC

sc-13133 FITC
200 µg/ml
$330.00

Vitamin D Receptor/VDR 항체 (D-6) PE

sc-13133 PE
200 µg/ml
$343.00

Vitamin D Receptor/VDR 항체 (D-6) Alexa Fluor® 488

sc-13133 AF488
200 µg/ml
$357.00

Vitamin D Receptor/VDR 항체 (D-6) Alexa Fluor® 546

sc-13133 AF546
200 µg/ml
$357.00

Vitamin D Receptor/VDR 항체 (D-6) Alexa Fluor® 594

sc-13133 AF594
200 µg/ml
$357.00

Vitamin D Receptor/VDR 항체 (D-6) Alexa Fluor® 647

sc-13133 AF647
200 µg/ml
$357.00

Vitamin D Receptor/VDR 항체 (D-6) Alexa Fluor® 680

sc-13133 AF680
200 µg/ml
$357.00

Vitamin D Receptor/VDR 항체 (D-6) Alexa Fluor® 790

sc-13133 AF790
200 µg/ml
$357.00

Vitamin D Receptor/VDR 항체 (D-6) B

sc-13133 B
200 µg/ml
$320.00

Can we use the PE conjugated antibody for Western? Is there an antibody that works for both Western blot and flow cytometry (not image based western).

Asked by: KRMBCM
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2023-03-13

This antibody can be used for immunocytochemistry?

Asked by: Anonymous
Thank you for your question. Vitamin D Receptor/VDR Antibody (D-6): sc-13133 is recommended for detection of VDR of mouse, rat and human origin by WB, IP, IF and IHC(P), so it is suitable for immunocytochemistry.
Answered by: Tech Support Europe
Date published: 2022-11-16

Wondering how the Alexa Fluor® 680 or Alexa Fluor® 790 conjugated antibody works for flow cytometry? Would be good to get a list of publications where it is specifically used for flow as the list of publications is lengthy...

Asked by: Halip5
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-03-18

Hi there, since the VDR has different isoforms, I am wondering the amino acids sequence for VDR this antibody corresponding. Thanks!

Asked by: Fay Liu
Thank you for your question. VDR Antibody (D-6): sc-13133 was raised against amino acids 344-424 of VDR isoform 1 of human origin. This is the corresponding amino acid sequence: PGVQDAA LIEAIQDRLS NTLQTYIRCR HPPPGSHLLY AKMIQKLADL RSLNEEHSKQ YRCLSFQPEC SMKLTPLVLE VFGN Please do not hesitate to contact our technical service directly if you have additional questions.
Answered by: Tech Support Europe
Date published: 2019-06-05

does D6 vitamin d receptor antibody cross react with monkey (macaques) for immunohistochemistry

Asked by: schristak
Thank you for your question. This antibody has not been tested on monkey samples in house, so it is not technically recommended or warrantied for that use, however, we do have publications in monkey (rhesus) samples.
Answered by: Tech Service
Date published: 2019-04-09

Hello, I have been working with another VDR and I am not certain I am fully appreciating that VDR. Hence I am interested in the "Contact us to receive a FREE 10 µg sample of VDR (D-6): sc-13133." offer of getting 10 ug for free. Is it possible?

Asked by: hanna abrahamsson
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-14

Can Santa Cruz provide VDR IHC images (performed using D-6 sc-13133 ab) with staining intensity (score) of 1 to 4 and % positive stained cells (score) with 1-4 (preferably in FFPE colorectal tumor tissue samples) ?

Asked by: Sanbc
Thank you for the question. Please note that this product is sold for research or laboratory use only and it must not been used for medical diagnostics. All available IHC(P) images for this product will be published upon availability on our product website. We currently show staining of human parathyroid gland tissue showing nuclear and cytoplasmic staining of glandular cells, human skin tissue showing nuclear and cytoplasmic staining of keratinocytes, fibroblasts, Langerhans cells and melanocytes and human adrenal gland tissue showing nuclear staining of cortical cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Answered by: Technical Support Europe
Date published: 2018-01-10

I got a double band when I used this antibody, any idea what the other band could be? Thanks

Asked by: Reham
Thank you very much for your inquiry. Please understand that we do not interpret customer data, since we do not have a detailed knowledge about your experiment and how the data was produced. Therefore our interpretation of the data could lead to a wrong direction without knowing all the relevant details. However, Western blotting is a technique that separates proteins based on size. In general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors, so the actual band size observed may differ from that predicted. Common factors include: 1. Post-translational modification - e.g. phosphorylation, glycosylation etc, which increases the size of the protein. 2. Post-translation cleavage - e.g. many proteins are synthesized as pro-proteins and then cleaved to give the active form, e.g. pro-caspases. 3. Splice variants - alternative splicing may create different sized proteins produced from the same gene. 4. Relative charge - the composition of amino acids (charged vs non-charged). 5. Multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.
Answered by: Technical Support Europe
Date published: 2021-05-09
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Rated 5 out of 5 by from 组化效果不错一个月前申请了试用装，用来做石蜡切片的组化效果确实可以的，考虑买个正装。如果正装价格还能有折扣就好了
Date published: 2024-04-19
Rated 1 out of 5 by from Cambio de loteEste anticuerpo funcionaba muy bien, pero el nuevo lote #A0621 no funciona ni para western-blot, ni para inmunoprecipitación ni para inmunofluorescencia
Date published: 2021-10-29
Rated 5 out of 5 by from Worked well in human and mouse muscle tissue.I used this antibody for IHC and got good results following published protocols. I used the unconjugated primary antibody and had to amplify for the human samples, but the mouse samples worked well going straight into secodary.
Date published: 2021-01-25
Rated 3 out of 5 by from low dilutionI needed to use a low dilution to make it work (1:50)
Date published: 2020-09-22
Rated 5 out of 5 by from Buen anticuerpoTiene una alta especificidad, en wblot da una muy buena señal y se puede reutilizar varias veces.
Date published: 2020-09-01
Rated 5 out of 5 by from very good antibodyThis antibody works very well in Wb. Also worked in IF in a PLA assay. Very satisfied.
Date published: 2019-01-02
Rated 5 out of 5 by from Best AntibodyWe have tried this antibody and published. Instruction and protocol was well described. I strongly recommend this antibody to other researcher.
Date published: 2018-08-24
Rated 5 out of 5 by from EXCELLENT SENSITIVITYTO SEE VDR ON PROTEIN LEVEL IS VERY TOUGH, BUT USING THE HYPEROSMOLAR BUFFER LYSIS AND THIS VDR ANTIBODY. I GOT THE BAND OF VDR. THIS ANTIBODY IS BETTER THAN ABCAM. THANK YOU
Date published: 2018-04-12
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Vitamin D Receptor/VDR 항체 (D-6) is rated 4.5 out of 5 by 32.
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