Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
USP22 Double Nickase Plasmid (m): sc-432127-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- USP22 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- USP22 Double Nickase Plasmid (m) and USP22 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Usp22. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: USP22 Antibody (C-3): sc-390585
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
USP22 Double Nickase Plasmid (m) | sc-432127-NIC | 20 µg | $410.00 | |||
USP22 Double Nickase Plasmid (m2) | sc-432127-NIC-2 | 20 µg | $410.00 |
Mouse Usp22 encodes USP22, a deubiquitinating enzyme within the SAGA transcriptional coactivator complex that removes ubiquitin from histone H2B and other substrates to modulate chromatin accessibility and gene expression programs. Through regulation of transcriptional initiation and elongation, USP22 influences cell-cycle progression, DNA damage responses, and differentiation-associated pathways. Altered USP22 activity has been linked to dysregulated transcriptional networks and proteostasis, making it relevant to studies of oncogenic signaling, stem-like phenotypes, and epigenetic control of tumor suppressor and proliferation genes. In mouse models, Usp22 perturbation is used to interrogate how ubiquitin-dependent chromatin remodeling shapes tissue homeostasis and disease-associated transcriptional states.
USP22 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Usp22 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Usp22. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Usp22 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Usp22-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.