USP22 Antibody (C-3): sc-390585

USP22 Antibody (C-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects USP22 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-USP22 antibody (C-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. USP22, also known as ubiquitin specific peptidase 22 or USP3L, is a 525 amino acid protein characterized by the presence of a UBP-type zinc finger, which is crucial for enzymatic activity. USP22 plays a significant role in the ubiquitin-proteasome pathway, a vital cellular mechanism that regulates protein degradation and turnover, thereby influencing various cellular processes such as signal transduction, transcriptional activation, and cell cycle progression. USP22 catalyzes the conversion of ubiquitin C-terminal thioesters to free ubiquitin and thiol, maintaining cellular homeostasis and potentially acting as a marker for cancer stem cells, highlighting potential importance in cancer biology. USP22 is encoded on human chromosome 17, a region encompassing over 2.5% of the human genome and responsible for encoding more than 1,200 genes, underscoring complexity and significance in various biological contexts.

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USP22 Antibody (C-3) References:

1. Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.  |  Kikuno, R., et al. 1999. DNA Res. 6: 197-205. PMID: 10470851
2. The expression patterns of deubiquitinating enzymes, USP22 and Usp22.  |  Lee, HJ., et al. 2006. Gene Expr Patterns. 6: 277-84. PMID: 16378762
3. A TFTC/STAGA module mediates histone H2A and H2B deubiquitination, coactivates nuclear receptors, and counteracts heterochromatin silencing.  |  Zhao, Y., et al. 2008. Mol Cell. 29: 92-101. PMID: 18206972
4. The putative cancer stem cell marker USP22 is a subunit of the human SAGA complex required for activated transcription and cell-cycle progression.  |  Zhang, XY., et al. 2008. Mol Cell. 29: 102-11. PMID: 18206973
5. USP22, an hSAGA subunit and potential cancer stem cell marker, reverses the polycomb-catalyzed ubiquitylation of histone H2A.  |  Zhang, XY., et al. 2008. Cell Cycle. 7: 1522-4. PMID: 18469533
6. USP22 promotes hypoxia-induced hepatocellular carcinoma stemness by a HIF1α/USP22 positive feedback loop upon TP53 inactivation.  |  Ling, S., et al. 2020. Gut. 69: 1322-1334. PMID: 31776228
7. USP22 regulates lipidome accumulation by stabilizing PPARγ in hepatocellular carcinoma.  |  Ning, Z., et al. 2022. Nat Commun. 13: 2187. PMID: 35449157
8. USP22 suppresses the NLRP3 inflammasome by degrading NLRP3 via ATG5-dependent autophagy.  |  Di, Q., et al. 2023. Autophagy. 19: 873-885. PMID: 35900990
9. PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells.  |  Li, Q., et al. 2022. Nat Commun. 13: 7677. PMID: 36509766
10. USP22 upregulates ZEB1-mediated VEGFA transcription in hepatocellular carcinoma.  |  Zeng, K., et al. 2023. Cell Death Dis. 14: 194. PMID: 36906615
11. EZH2 Inhibition Enhances PD-L1 Protein Stability Through USP22-Mediated Deubiquitination in Colorectal Cancer.  |  Huang, J., et al. 2024. Adv Sci (Weinh). 11: e2308045. PMID: 38520088
12. The p-MYH9/USP22/HIF-1α axis promotes lenvatinib resistance and cancer stemness in hepatocellular carcinoma.  |  Shan, Q., et al. 2024. Signal Transduct Target Ther. 9: 249. PMID: 39300073

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-USP22 Antibody (C-3) has 15 citations in a variety of scientific publications.

Primary antibodies like Anti-USP22 Antibody (C-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

USP22 Antibody (C-3) Specifications

Alternate Product Name: USP22

Product Clone Name: C-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 130-176

Epitope Target: USP22

Epitope Species: human

Product Reactivity/Detection: USP22

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine and bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 15 citations in various scientific publications

Available Conjugates/Forms for USP22 Antibody (C-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin