Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TTP Double Nickase Plasmid (h): sc-400722-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TTP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TTP Double Nickase Plasmid (h) and TTP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ZFP36. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TTP Antibody (A-8): sc-374305
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TTP Double Nickase Plasmid (h) | sc-400722-NIC | 20 µg | $410.00 | |||
TTP Double Nickase Plasmid (h2) | sc-400722-NIC-2 | 20 µg | $410.00 |
Human ZFP36 encodes tristetraprolin (TTP), an AU-rich element–binding RNA-binding protein that promotes deadenylation and decay of labile mRNAs, thereby constraining expression of cytokines and growth-associated transcripts. TTP integrates post-transcriptional control with inflammatory signaling pathways, including TNF/NF-κB and MAPK-regulated phosphorylation that modulates its RNA-binding activity and subcellular localization. By shaping transcript stability programs, TTP influences macrophage activation, stress responses, and cell-cycle progression. Altered ZFP36/TTP expression or function has been linked to dysregulated inflammation and tumor-associated gene-expression signatures, making it a relevant target for mechanistic studies of immune and oncogenic networks.
TTP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ZFP36 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ZFP36. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ZFP36 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ZFP36-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.