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TTP Antibody (A-8) | SCBT - Santa Cruz Biotechnology
TTP 항체 (A-8) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 TTP을 검출할것을 권장합니다.

TTP 항체 (A-8): sc-374305

TTP 항체 (A-8) is rated 3.6 out of 5 by 10.
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데이터 시트

    • TTP 항체 A-8 는 마우스 monoclonal IgG2a κ TTP 항체, 14간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • human origin의 TTP에서 C-terminus에 위치한 166-285 아미노산을 항원으로 사용하였습니다.
    • TTP 항체 (A-8)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 TTP 를 감지하는 데에 추천한다.
    • Anti-TTP Antibody (A-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • TTP (A-8): sc-374305무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TTP Antibody (A-8) for WB and IHC(P) applications. These reagents are now offered in bundles with TTP Antibody (A-8) (see ordering information below).
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Could you advise whether this antibody has been tested in house in western blot for human endothelial cells please? Also, what's the ideal amount of protein/cell lysate required to get a good signal/band? 

Asked by: Cass
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Anonymous
Date published: 2022-05-26

Can TTP (A-8): sc-374305 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jenniferc
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, TTP (A-8): sc-374305 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Nonspecific This antibody still detected true same bands in a KO mouse sample!
Date published: 2017-11-17
Rated 3 out of 5 by from Good for WB There is a band in WB using mouse BMDM. But there is also a non-specific band about 70 kD.
Date published: 2017-09-28
Rated 4 out of 5 by from Decent staining in mouse bone marrow-derived DCs Loaded protein from ~500k lysed BMDC, blocked blots with milk and, incubated in anti-TTP Ab (1:500 in 5% BSA in TBS-T) overnight and used another company's HRP anti-mouse secondary. Got three bands shown in attached image: the top band is at the 49kDa marker, the bottom (very faint) band is at about the 38kDa marker.
Date published: 2017-08-21
Rated 5 out of 5 by from Good result Loading 40 ug protein extract from HePG2 cell ,antibody dilution (dilution 1/500 as recommended).The antibody recognizes a single band of expected size at 44kda,very strong signal.
Date published: 2017-07-29
Rated 1 out of 5 by from TTP (A-8) This antibody didn't work in western blot to detect the endogenous TTP in human cell lines.
Date published: 2017-07-24
Rated 4 out of 5 by from Great antibody! I used this antibody for immunoblotting. There is a clear band. 1:500 dilution is sufficient and can be used many times
Date published: 2017-06-05
Rated 5 out of 5 by from cytoplasmic staining Good cytoplasmic staining with paraffin-embedded human breast samples.I would recommend to others
Date published: 2017-05-27
Rated 4 out of 5 by from GOOD Good specificity in WB in non-transfected A549 cells.
Date published: 2016-11-29
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