human origin의 TICAM-1에서 N-terminus에 위치한 1-272 아미노산을 항원으로 사용하였습니다.
안티-TICAM-1 항체 (E-7)는 WB, IP, IF and ELISA으로 mouse, rat and human유래의 TICAM-1 를 감지하는 데에 추천한다.
IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-TICAM-1 항체 (E-7)를 제공한다.
WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for TICAM-1 Antibody (E-7) for WB applications. These reagents are now offered in bundles with TICAM-1 Antibody (E-7) (see ordering information below).
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TICAM-1 Antibody (E-7) is a high quality monoclonal TICAM-1 antibody (also designated TICAM1 antibody or TRIF antibody) suitable for the detection of the TICAM-1 protein of mouse, rat and human origin. TICAM-1 Antibody (E-7) is available as both the non-conjugated anti-TICAM-1 antibody form, as well as multiple conjugated forms of anti-TICAM-1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Toll/interleukin-1 receptor/resistance (TIR) adaptor protein (Trif, TICAM-1) can physically bind TIR domains and influence cell signaling. TICAM-1 interacts with TLR3 and mediates dsRNA activation of interferon-beta, through NFkappaB, AP1, or IRF3. Human TICAM-1 maps to chromosome 19p13.3.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What is the recommended starting dilution for Western blot for sc-514384: TICAM-1 (E-7) monoclonal antibody?
Asked by: Professor Griffin
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: