TCR delta 항체 (H-41) WB, IP 와ELISA으로 human origin의 TCR δ을 검출할것을 권장합니다.

TCR δ 항체 (H-41): sc-100289

TCR δ 항체 (H-41) is rated 4.6 out of 5 by 5.
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Datasheets

    • 안티-TCR delta 항체 H-41 는 마우스 monoclonal IgG1 (kappa light chain) TCR δ 항체 이며 100 µg/ml으로 제공합니다.
    • human origin의 TCR δ의 전체인 173-273 아미노산을 항원으로 사용하였습니다.
    • WB, IP 와ELISA으로 human origin의 TCR δ을 검출할것을 권장합니다.
    • 강력추천하는 conventional secondary anti-mouse IgG reagents의 대체제품 보기m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP).
    • TCR δ (H-41): sc-100289무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service 11
Date published: 2017-02-03
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Rated 5 out of 5 by from Very good TCRgd+ cell staining on FFPE After TCR gamma.20 (from Thermo Scientific) being discontinued, finally I've found in Santa Cruz Biotechnology TCR delta a solid antibody useful to recognize TCRgd cells in formalin-fixed paraffin embedded sample using immunohistochemistry. I've stained sections from tonsil and small intestine with a range of dilution from 1:25 to 1:250 and I've achieved strong and tidy stainings in all dilutions tested. Also antigen unmasking is very tolerant. It works using EDTA pH8.0 or Citrate pH 6.0 in microwave or water bath. I'm very satisfied from the results
Date published: 2020-03-12
Rated 5 out of 5 by from Excellent staining for IHC on FFPE tissues I successfully used this product for immunohistochemistry on 4 micron sections of FFPE tonsil tissues and FFPE cell block of a bone marrow blood clot. I got excellent results using 1:50-1:100 dilution for 1 hour incubation at room temperature manually or using the Ventena Benchmark Ultra autostainer. The best results were obtained using EDTA pH9 in a steamer then placed on the autostainer, or alternately using CC1 at 99 degrees C for 36 minutes. Ventena Ultraview/DAB was used for the detection system.
Date published: 2018-06-19
Rated 5 out of 5 by from Clean and Great expression of TCR Delta. I struggled for over a year to find a replacement for TCR Gamma, which was discontinued by Thermo last year. This is a great replacement for it. I can't upload a TIFF image, but the examples that I wanted to upload were from a Spleen and Colon. The retrieval for Leica Bond III was Hier2 for 40 minutes with 15 minutes of antibody incubation (modified F Protocol). Dilution that worked best was 1:200 (using Leica's diluent).
Date published: 2017-12-20
Rated 5 out of 5 by from Good for human detection Positive signal obtained at predicted molecular weight in WB for TCR delta.
Date published: 2017-02-07
Rated 3 out of 5 by from Faint band at correct MW in HeLa whole cell lysate Faint band at correct MW in HeLa whole cell lysate. -SCBT QC
Date published: 2013-12-12
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