Smad1 항체 (A-4) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat, human와 mink origin의 Smad1을 검출할것을 권장합니다.

Smad1 항체 (A-4): sc-7965

Smad1 항체 (A-4) is rated 4.9 out of 5 by 16.
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데이터 시트

    • 안티-Smad1 항체 A-4 는 마우스 monoclonal IgG1 κ Smad1 항체, 105간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • 아미노 산1-465 representing full length Smad1 of human origin
    • 안티-Smad1 항체 (A-4)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat, human and mink유래의 Smad1 를 감지하는 데에 추천한다.
    • IP를 위해 agarose;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC에 결합된 Smad1 항체 (A-4)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-7965 X, 200 µg/0.1 ml)이 있습니다.
    • Smad1 (A-4): sc-7965무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP is the preferred secondary detection reagent for Smad1 Antibody (A-4) for WB and IHC(P) applications. This reagent is now offered in a bundle with Smad1 Antibody (A-4) (see ordering information below).
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Does the antibody cross-react with Smad5 and Smad8(9)?

Asked by: Jessica22
Thank you for your question. This mouse monoclonal antibody is specific for Smad1 and does not cross-react with Smad5 or Smad8(9).
Answered by: Technical Support
Date published: 2020-07-07

Can this anti-body be used in IP test of zebrafish?

Asked by: Paul111
Thank you for your question. We have not yet tested this antibody in zebrafish samples. There is one known product citation using this antibody for WB/IF in zebrafish samples. The reference number for that publication is PMID: 24777107.
Answered by: Technical Support
Date published: 2020-05-18

Can this antibody be used in WB test of rabbit bone tissue?Thank you.

Asked by: jack35
Thank you for your question. This antibody does not have data or any product citations in rabbit samples at this time. Please contact our Technical Service Department if you would like our assistance in checking the antibody's antigen sequence for similarity with the Smad1 protein in rabbits.
Answered by: Technical Support
Date published: 2020-04-20

What's the buffer composition for this antibody?

Asked by: azam5811
Thank you for your question. The standard format of this antibody is provided in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. We also provide a more concentrated carrier free version (without gelatin) in PBS + sodium azide alone, under the "X" catalog number, sc-7965 X, 200 µg/0.1 ml.
Answered by: Technical Support
Date published: 2020-04-17

I am using Smad1 (A-4): sc-7965 to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 10
Date published: 2017-01-13

Is there a blocking peptide available for Smad1 (A-4): sc-7965?

Asked by: cjMara
No. Smad1 Antibody (A-4) is a mouse monoclonal raised against amino acids 1-465 representing full length Smad1 of human origin. There is no blocking peptide available.
Answered by: Technical Support 10
Date published: 2017-01-13
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Rated 5 out of 5 by from Good results Works great for WB, It always gave us specific results with strong signal
Date published: 2017-09-12
Rated 4 out of 5 by from 很不错 1:200稀释抗体,检测肥大软骨细胞中Smad1的表达量,发现条带很明显,达到了预期效果,十分感谢试用装的馈赠
Date published: 2017-09-11
Rated 5 out of 5 by from Worked well I had great results using this antibody at 1:500 dilution.
Date published: 2017-06-21
Rated 5 out of 5 by from It worked well IHC staining human stomach tissue got strong positive staining, the staining intensity between these cells was obvious
Date published: 2017-04-30
Rated 5 out of 5 by from Great antibody I am happy I purchase this antibody. It was very stable and the result could be repeated.I will continue to use it.
Date published: 2017-02-13
Rated 5 out of 5 by from target band seen It's worked well in WB with rat kidney tissue lysates, I got the target band .
Date published: 2017-02-11
Rated 5 out of 5 by from Outstanding Nice product for IHC staining applications; also strong WB
Date published: 2017-01-23
Rated 5 out of 5 by from Great results in IHC This Smad1 antibody shows good results in IHC for human and rat samples.
Date published: 2017-01-14
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