RelB Antibody (D-4): sc-48366

RelB Antibody (D-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects RelB protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-RelB antibody (D-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RelB protein plays a crucial role in the regulation of immune responses and inflammation, functioning as a key component of the NF-κB signaling pathway. This pathway is vital for the transcriptional activation of genes involved in immune and inflammatory responses, making RelB essential for maintaining immune homeostasis and responding to pathogens. Notably, RelB is predominantly located in the nucleus, where RelB forms heterodimers with p50, enhancing RelB′s ability to transactivate genes with κB binding sites. The interaction between RelB and p50 is particularly important because this interaction allows for a more nuanced regulation of gene expression compared to homodimers, thereby influencing various biological processes such as cell survival, proliferation, and differentiation. Anti-RelB antibody (D-4) versatility in multiple applications makes this reagent an invaluable tool for researchers studying the complexities of immune regulation and the underlying mechanisms of various diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

RelB Antibody (D-4) References:

1. RelB, a new Rel family transcription activator that can interact with p50-NF-kappa B.  |  Ryseck, RP., et al. 1992. Mol Cell Biol. 12: 674-84. PMID: 1732739
2. Effector and regulatory T-cell function is differentially regulated by RelB within antigen-presenting cells during GVHD.  |  MacDonald, KP., et al. 2007. Blood. 109: 5049-57. PMID: 17327399
3. RelB is the NF-kappaB subunit downstream of NIK responsible for osteoclast differentiation.  |  Vaira, S., et al. 2008. Proc Natl Acad Sci U S A. 105: 3897-902. PMID: 18322009
4. Cloning of an NF-kappa B subunit which stimulates HIV transcription in synergy with p65.  |  Schmid, RM., et al. 1991. Nature. 352: 733-6. PMID: 1876189
5. Cloning of the p50 DNA binding subunit of NF-kappa B: homology to rel and dorsal.  |  Ghosh, S., et al. 1990. Cell. 62: 1019-29. PMID: 2203532
6. NF-kappa B, KBF1, dorsal, and related matters.  |  Gilmore, TD. 1990. Cell. 62: 841-3. PMID: 2203533
7. Cloning of a mitogen-inducible gene encoding a kappa B DNA-binding protein with homology to the rel oncogene and to cell-cycle motifs.  |  Bours, V., et al. 1990. Nature. 348: 76-80. PMID: 2234062
8. A 65-kappaD subunit of active NF-kappaB is required for inhibition of NF-kappaB by I kappaB.  |  Baeuerle, PA. and Baltimore, D. 1989. Genes Dev. 3: 1689-98. PMID: 2691328
9. Multiple nuclear factors interact with the immunoglobulin enhancer sequences.  |  Sen, R. and Baltimore, D. 1986. Cell. 46: 705-16. PMID: 3091258
10. RelB is a potential molecular biomarker for immunotherapy in human pan-cancer.  |  Wu, J., et al. 2023. Front Mol Biosci. 10: 1178446. PMID: 37388242
11. RelB-deficient autoinflammatory pathology presents as interferonopathy, but in mice is interferon-independent.  |  Navarro, HI., et al. 2023. J Allergy Clin Immunol. 152: 1261-1272. PMID: 37460023
12. Inherited human RelB deficiency impairs innate and adaptive immunity to infection.  |  Le Voyer, T., et al. 2024. Proc Natl Acad Sci U S A. 121: e2321794121. PMID: 39231201

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RelB Antibody (D-4) has 33 citations in a variety of scientific publications.

Primary antibodies like Anti-RelB Antibody (D-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RelB Antibody (D-4) Specifications

Alternate Product Name: RelB

Product Clone Name: D-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 380-579

Epitope Target: RelB

Epitope Species: human

Product Reactivity/Detection: RelB

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 33 citations in various scientific publications

Available Conjugates/Forms for RelB Antibody (D-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent