Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PYK2 Double Nickase Plasmid (h): sc-400708-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PYK2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PYK2 Double Nickase Plasmid (h) and PYK2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PTK2B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PYK2 Antibody (E-3): sc-393181
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PYK2 Double Nickase Plasmid (h) | sc-400708-NIC | 20 µg | $410.00 | |||
PYK2 Double Nickase Plasmid (h2) | sc-400708-NIC-2 | 20 µg | $410.00 |
Human PTK2B encodes the non-receptor tyrosine kinase PYK2, a focal adhesion–associated signaling node activated downstream of integrins, growth factor receptors, chemokine receptors, and Ca2+/PKC inputs. PYK2 coordinates phosphorylation cascades involving Src family kinases, FAK, MAPK/ERK, and PI3K-AKT to regulate cytoskeletal remodeling, adhesion turnover, migration, and vesicle trafficking. It also contributes to innate immune and inflammatory signaling, including pathways linked to leukocyte activation and microglial responses. Dysregulated PTK2B/PYK2 signaling has been associated with altered cell motility and survival programs relevant to cancer biology and with genetic and functional links to neuroinflammation and neurodegenerative disease mechanisms.
PYK2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PTK2B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PTK2B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PTK2B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PTK2B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.