Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Plectin Double Nickase Plasmid (h): sc-401494-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Plectin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Plectin Double Nickase Plasmid (h) and Plectin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PLEC. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Plectin Antibody (10F6): sc-33649
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Plectin Double Nickase Plasmid (h) | sc-401494-NIC | 20 µg | $410.00 | |||
Plectin Double Nickase Plasmid (h2) | sc-401494-NIC-2 | 20 µg | $410.00 |
PLEC encodes plectin, a large cytolinker protein that integrates intermediate filaments with actin, microtubules, and membrane-associated complexes to stabilize cell architecture under mechanical stress. Plectin organizes hemidesmosomes, focal adhesions, and desmosome-associated networks, supporting epithelial integrity, muscle fiber resilience, and neuronal cytoskeletal organization. Through these scaffolding functions, plectin influences processes including cell adhesion, migration, mechanotransduction, and maintenance of cytoskeletal dynamics. Dysregulation or mutation of PLEC is associated with disorders affecting skin and muscle integrity and has been investigated in the context of cancer cell invasion and altered tissue mechanics.
Plectin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PLEC locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PLEC. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PLEC function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PLEC-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.