PBEF 항체 (E-3) WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 PBEF을 검출할것을 권장합니다.

PBEF 항체 (E-3): sc-393444

PBEF 항체 (E-3) is rated 5.0 out of 5 by 13.
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Datasheets

    • 안티-PBEF 항체 E-3 는 마우스 monoclonal IgG2b (kappa light chain) PBEF 항체 이며 200 µg/ml으로 제공합니다.
    • human origin의 PBEF에서 N-terminus에 위치한 1-300 아미노산을 항원으로 사용하였습니다.
    • 안티-PBEF 항체 (E-3)는 WB, IP, IF and ELISA으로 mouse, rat and human유래의 PBEF 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-PBEF 항체 (E-3)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • 강력추천하는 conventional secondary anti-mouse IgG reagents의 대체제품 보기m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP).
    • PBEF (E-3): sc-393444무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.

What is the recommended fixation method for immunofluorescence staining with PBEF (E-3): sc-393444 monoclonal antibody?

Asked by: AbPolly
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Very good antibody I used this antibody in mouse liver and muscle samples. This antibody works well in both tissues. I was able to get clean bands in WB with 1:500 dilution (by both fluorescence and chemiluminescence).
Date published: 2020-07-27
Rated 5 out of 5 by from Works well Probed at 1:500 overnight, detected strong bands at the right size
Date published: 2017-11-15
Rated 5 out of 5 by from Satisfied with the antibody PBEF antibody provides great staining in IF and IHC applications, binding is both strong and specific.The antibody could be performed under isothermal conditions, its specificity was good and the sensitivity.
Date published: 2017-10-09
Rated 5 out of 5 by from This antibody is very good. PBEF antibody works well for WB at the concentration of 1:200, with satisfied bands.
Date published: 2017-05-23
Rated 5 out of 5 by from Excellent for WB it works excellent for WB in our lab, will keep usinig it
Date published: 2017-05-08
Rated 5 out of 5 by from Excellent antibody! I apply for the sample sack of this antibody,the band is special and clear.
Date published: 2017-03-29
Rated 5 out of 5 by from The Product is Good We tried it detect this sample from dilution 1:800. The researchers confirmed that PBEF Antibody accurately detects rat with high sensitivity and specificity. We were convinced the antibody.
Date published: 2017-02-13
Rated 5 out of 5 by from WB and worked fine This gave very strong signal in our Western, we use dilution to 1:400 and it worked well.
Date published: 2017-02-12
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