Date published: 2026-10-18
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PABP Antibody (F-2) - Western Blotting - Image 354945
PABP Antibody (F-2): sc-166027. Western blot analysis of PABP expression in A549 (A), NIH/3T3 (B), MCF7 (C), Hep G2 (D) and NTERA-2 cl.D1 (E) whole cell lysates.
PABP Antibody (F-2): sc-166027. Western blot analysis of PABP expression in A549 (A), NIH/3T3 (B), MCF7 (C), Hep G2 (D) and NTERA-2 cl.D1 (E) whole cell lysates.

PABP Antibody (F-2): sc-166027

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Datasheets
  • PABP Antibody (F-2) is a mouse monoclonal IgG2a κ, cited in 8 publications, provided at 200 µg/ml
  • raised against amino acids 1-300 deletion 82-129 (deletion 82-129) mapping at the N-terminus of PABP of human origin
  • recommended for detection of PABP1, PABP2, PABP3, PABP4 and PABP5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
  • See PABP (A-4): sc-166381 for PABP antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for PABP Antibody (F-2) for WB and IHC(P) applications. These reagents are now offered in bundles with PABP Antibody (F-2) (see ordering information below).
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PABP Antibody (F-2) is a mouse monoclonal IgG2a antibody that detects Poly(A)-binding protein (PABP) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PABP plays a crucial role in gene expression by binding to the poly(A) tail of eukaryotic mRNA, which is essential for mRNA stability, translation initiation, and regulation of mRNA degradation. PABP′s interaction with eukaryotic translation initiation factor 4G (eIF4G) facilitates assembly of translation initiation complex, promoting efficient translation of polyadenylated mRNAs. PABP′s modular structure, characterized by four N-terminal RNA-binding domains and a large C-terminus, allows for versatile interactions with various RNA sequences and proteins, influencing mRNA fate and cellular responses. During viral infections, such as poliovirus, PABP cleavage can lead to shutdown of host protein synthesis, highlighting PABP′s significance in both normal cellular function and viral pathogenesis. PABP gene is located on chromosome 8q22.2-q23 and encodes a protein consisting of 633 amino acids, underscoring PABP′s evolutionary conservation and essential role in cellular processes.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

PABP Antibody (F-2) References:

  1. The mRNA closed-loop model: the function of PABP and PABP-interacting proteins in mRNA translation.  |  Kahvejian, A., et al. 2001. Cold Spring Harb Symp Quant Biol. 66: 293-300. PMID: 12762031
  2. Human PABP binds AU-rich RNA via RNA-binding domains 3 and 4.  |  Sladic, RT., et al. 2004. Eur J Biochem. 271: 450-7. PMID: 14717712
  3. Regulation of poly(A)-binding protein through PABP-interacting proteins.  |  Derry, MC., et al. 2006. Cold Spring Harb Symp Quant Biol. 71: 537-43. PMID: 17381337
  4. Modulation of enteroviral proteinase cleavage of poly(A)-binding protein (PABP) by conformation and PABP-associated factors.  |  Rivera, CI. and Lloyd, RE. 2008. Virology. 375: 59-72. PMID: 18321554
  5. PABP enhances release factor recruitment and stop codon recognition during translation termination.  |  Ivanov, A., et al. 2016. Nucleic Acids Res. 44: 7766-76. PMID: 27418677
  6. Translation of insulin granule proteins are regulated by PDI and PABP.  |  Sarwade, RD., et al. 2020. Biochem Biophys Res Commun. 526: 618-625. PMID: 32248978
  7. De novo variants in the PABP domain of PABPC1 lead to developmental delay.  |  Wegler, M., et al. 2022. Genet Med. 24: 1761-1773. PMID: 35511136
  8. Chemically Modified Poly(A) Analogs Targeting PABP: Structure Activity Relationship and Translation Inhibitory Properties.  |  Perzanowska, O., et al. 2022. Chemistry. 28: e202201115. PMID: 35575378
  9. Short poly(A) tails are protected from deadenylation by the LARP1-PABP complex.  |  Park, J., et al. 2023. Nat Struct Mol Biol. 30: 330-338. PMID: 36849640
  10. Poised PABP-RNA hubs implement signal-dependent mRNA decay in development.  |  Modic, M., et al. 2024. Nat Struct Mol Biol. 31: 1439-1447. PMID: 39054355

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

PABP Antibody (F-2)

sc-166027
200 µg/ml
$322.00

PABP Antibody (F-2): m-IgG Fc BP-HRP Bundle

sc-539992
200 µg Ab; 10 µg BP
$361.00

PABP Antibody (F-2): m-IgG2a BP-HRP Bundle

sc-546831
200 µg Ab; 10 µg BP
$361.00

I am using PABP (F-2): sc-166027 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by Dewen_You from I used this antibody for WBI used this antibody for WB. The bands are beautiful.
Date published: 2015-09-30
Rated 5 out of 5 by SS from Good publishable data in CoIP in human HeLa cellsGood publishable data in CoIP in human HeLa cells. -SCBT Publication Review
Date published: 2015-05-28
Rated 5 out of 5 by LR from Strong band in WB in PCStrong band in WB in PC-3 whole cell lysate. -SCBT QC
Date published: 2015-05-22
Rated 5 out of 5 by LF from Excellent immunoperoxidase cytoplasmic stainingExcellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human colon tissue. -SCBT QC
Date published: 2015-05-20
Rated 5 out of 5 by QZ from Good specificity in WB in human transfectedGood specificity in WB in human transfected 293T and NTERA-2 cl.D1 whole cell lysates. -SCBT QC
Date published: 2015-03-12
Rated 5 out of 5 by CJ from Strong staining in IF in monkey vero cellsStrong staining in IF in monkey vero cells. -SCBT Publication Review
Date published: 2015-02-26
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PABP Antibody (F-2) is rated 5.0 out of 5 by 6.
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