p21 Waf1/Cip1/CDKN1A 항체 (F-8) WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 p21 Waf1/Cip1을 검출할것을 권장합니다.

p21 Waf1/Cip1/CDKN1A 항체 (F-8): sc-271610

p21 Waf1/Cip1/CDKN1A 항체 (F-8) is rated 4.5 out of 5 by 13.
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데이터 시트

    • p21 Waf1/Cip1/CDKN1A 항체(F-8) 는 마우스 monoclonal IgG2aκ, 31간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • 항원결정부는 human origin의 p21의 내부에 위치한 124-164의 아미노산에 해당됩니다.
    • 안티-p21 Waf1/Cip1/CDKN1A 항체 (F-8)는 WB, IP, IF and ELISA으로 mouse, rat and human유래의 p21 Waf1/Cip1 를 감지하는 데에 추천한다.; 이외에, canine and porcine등 species와 반응할수 있습니다
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-p21 Waf1/Cip1/CDKN1A 항체 (F-8)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • p21 Waf1/Cip1/CDKN1A (F-8): sc-271610무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for p21 Waf1/Cip1/CDKN1A Antibody (F-8) for WB applications. These reagents are now offered in bundles with p21 Waf1/Cip1/CDKN1A Antibody (F-8) (see ordering information below).
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What is the best secondary to use with your mouse monoclonal antibodies for WB?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 4
Date published: 2017-01-17
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Rated 5 out of 5 by from Worked excellent on our western blots We used it 1:1000 on our immunoblots. The signals were excellent.
Date published: 2021-07-29
Rated 1 out of 5 by from very bad product it is not working in cell lysate especially human cell lines (hepG2)
Date published: 2020-10-22
Rated 5 out of 5 by from Great antibody! Used at 1:200 for mouse tissue lysates. Antibody gave good clear band! Recommend!
Date published: 2018-03-23
Rated 5 out of 5 by from very strong signal Specific WB band in HeLa whole cell lysate, 1:300 dilution, Good data!!!
Date published: 2017-04-05
Rated 5 out of 5 by from Strong and Specific p21 (F-8) gives a strong specific band for Western blot (WB) at 21 kDa.
Date published: 2016-12-20
Rated 5 out of 5 by from great 1:1000 dilution for detection of p21 in DLBCL lymphoma cells, incubate with the filter overnight at 4C. It's very sensitive and stable with the clean background.
Date published: 2016-12-17
Rated 4 out of 5 by from This has worked well in WB with mouse liver This has worked well in WB with mouse liver.
Date published: 2015-12-07
Rated 5 out of 5 by from This has worked well in WB with mouse liver This has worked well in WB with mouse liver
Date published: 2015-10-16
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