p-Stat3 항체 (9E12) WB, IP와 IF으로 mouse, rat, human와 canine origin의 Tyr 705 phosphorylated Stat3을 검출할것을 권장합니다.

p-Stat3 항체 (9E12): sc-81523

p-Stat3 항체 (9E12) is rated 4.0 out of 5 by 5.
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데이터 시트

    • p-Stat3 항체 9E12 는 마우스 monoclonal IgG1, 21간행물에 인용, 이며 50 µg/0.5 ml으로 제공합니다.
    • raised against a synthetic phosphopeptide corresponding to the region surrounding p-Tyr 705 of Stat3 of human origin
    • WB, IP와 IF으로 mouse, rat, human와 canine origin의 Tyr 705 phosphorylated Stat3을 검출할것을 권장합니다.
    • See p-Stat3 (B-7): sc-8059 for additional antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • p-Stat3 (9E12): sc-81523무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for p-Stat3 Antibody (9E12) for WB applications. These reagents are now offered in bundles with p-Stat3 Antibody (9E12) (see ordering information below).
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I have read few people mentioned it worked very well for western. Could you tell me the dilution and is it in milk or 5%BSA incubation? Thanks

Asked by: mkunni
Thank you for your question. We recommend a starting dilution of 1:200 for Western blotting (range 1:100-1:1,000). We recommend using either 5% nonfat milk + TBST, or a mixture of 1% nonfat milk + 1% BSA in TBST for blocking and incubating both primary and secondary antibodies.
Answered by: Technical Support
Date published: 2019-06-12

For Western Blot, is it recommended to use denatured or non-denatured conditions with p-Stat3 (9E12): sc-81523 antibody?

Asked by: Professor Griffin
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 1 out of 5 by from No detección de banda No se detectó ninguna banda. Se probó el anticuerpo con diferentes cantidades (hasta 100ug) de proteínas extraídas de muestras de tejido.
Date published: 2018-01-03
Rated 5 out of 5 by from This product works great in WB This antibody worked really well for us in our Western, thought it was very stable.
Date published: 2017-05-07
Rated 5 out of 5 by from Nice results Nice and specific Western blot results in various samples with p-Stat3 Antibody (9E12)
Date published: 2017-01-27
Rated 4 out of 5 by from Works well for Western blot Works well for Western blot, strong band detected in IFN- -treated SK-MEL-28 whole cell lysates. -SCBT QC
Date published: 2015-03-12
Rated 5 out of 5 by from Great antibody for Western Great antibody for Western, strong band detected in IFN treated HeLa whole cell lysate. -SCBT QC
Date published: 2014-04-14
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