p-GSK3 beta Antibody (F-2): sc-373800

p-GSK-3β Antibody (F-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ser 9 phosphorylated GSK-3β in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). p-GSK-3β (F-2) antibody is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GSK-3β, a serine/threonine kinase, regulates various cellular processes, including glycogen metabolism and cell signaling pathways. GSK-3β activity is regulated by phosphorylation at specific serine and tyrosine residues, which can either inhibit or activate function. GSK-3β regulates transcription factors and metabolic enzymes, making GSK-3β essential for processes such as glycogen synthesis and cell adhesion. Phosphorylation of GSK-3β at Ser 9, detected by anti-GSK-3β antibody (F-2), inactivates the kinase, thereby modulating cellular signaling and preventing apoptosis in response to tumor necrosis factor-alpha (TNFα). GSK-3β interacts with various proteins, including cAMP-dependent protein kinase A, which phosphorylates and contributes to inactivation, highlighting intricate regulatory mechanisms. p-GSK-3β (F-2) monoclonal antibody serves as an invaluable tool for researchers studying complex signaling pathways involving GSK-3β and implications in various biological processes and diseases.

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p-GSK3 beta Antibody (F-2) References:

1. Requirement for glycogen synthase kinase-3beta in cell survival and NF-kappaB activation.  |  Hoeflich, KP., et al. 2000. Nature. 406: 86-90. PMID: 10894547
2. Phosphorylation and inactivation of glycogen synthase kinase 3 by protein kinase A.  |  Fang, X., et al. 2000. Proc Natl Acad Sci U S A. 97: 11960-5. PMID: 11035810
3. Glycogen synthase kinase-3: functions in oncogenesis and development.  |  Plyte, SE., et al. 1992. Biochim Biophys Acta. 1114: 147-62. PMID: 1333807
4. Curcumin ameliorates scopolamine-induced mice memory retrieval deficit and restores hippocampal p-Akt and p-GSK-3β.  |  SoukhakLari, R., et al. 2018. Eur J Pharmacol. 841: 28-32. PMID: 30321530
5. Glycogen synthase kinase-3 beta is a dual specificity kinase differentially regulated by tyrosine and serine/threonine phosphorylation.  |  Wang, QM., et al. 1994. J Biol Chem. 269: 14566-74. PMID: 7514173
6. Mitogen inactivation of glycogen synthase kinase-3 beta in intact cells via serine 9 phosphorylation.  |  Stambolic, V. and Woodgett, JR. 1994. Biochem J. 303 (Pt 3): 701-4. PMID: 7980435
7. Tyrosine dephosphorylation of glycogen synthase kinase-3 is involved in its extracellular signal-dependent inactivation.  |  Murai, H., et al. 1996. FEBS Lett. 392: 153-60. PMID: 8772194
8. Further evidence that the inhibition of glycogen synthase kinase-3beta by IGF-1 is mediated by PDK1/PKB-induced phosphorylation of Ser-9 and not by dephosphorylation of Tyr-216.  |  Shaw, M., et al. 1997. FEBS Lett. 416: 307-11. PMID: 9373175
9. Essential role for protein kinase B (PKB) in insulin-induced glycogen synthase kinase 3 inactivation. Characterization of dominant-negative mutant of PKB.  |  van Weeren, PC., et al. 1998. J Biol Chem. 273: 13150-6. PMID: 9582355
10. The activation of glycogen synthase by insulin switches from kinase inhibition to phosphatase activation during adipogenesis in 3T3-L1 cells.  |  Brady, MJ., et al. 1998. J Biol Chem. 273: 14063-6. PMID: 9603900

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-GSK3 beta Antibody (F-2) has 103 citations in a variety of scientific publications.

Primary antibodies like Anti-p-GSK3 beta Antibody (F-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-GSK3 beta Antibody (F-2) Specifications

Alternate Product Name: GSK3

Product Clone Name: F-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Ser 9

Epitope Target: GSK-3β

Epitope Species: human

Product Reactivity/Detection: Ser 9 phosphorylated GSK-3β

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 103 citations in various scientific publications

Available Conjugates/Forms for p-GSK3 beta Antibody (F-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred GSK3 alpha/beta antibody is GSK3 alpha/beta Antibody (0011-A): sc-7291.