MTO1 Antibody (H-5): sc-398386
- MTO1 Antibody (H-5) is a mouse monoclonal IgG1 κ MTO1 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 267-282 within an internal region of MTO1 of human origin
- MTO1 Antibody (H-5) is recommended for detection of MTO1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine and bovine
- Anti-MTO1 Antibody (H-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for MTO1 Antibody (H-5) for WB applications. These reagents are now offered in bundles with MTO1 Antibody (H-5) (see ordering information below).
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MTO1 Antibody (H-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects MTO1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MTO1 Antibody (H-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MTO1, also known as mitochondrial translation optimization 1 or CGI-02, is a 717 amino acid mitochondrial protein that plays a crucial role in mitochondrial tRNA modification, specifically in the 5-carboxymethylaminomethyl modification of wobble uridine bases. This modification is essential for accurate translation of mitochondrial mRNA, which is vital for proper functioning of mitochondria, the powerhouse of the cell. MTO1 is expressed ubiquitously, with highest levels found in tissues that have elevated metabolic rates, such as heart and skeletal muscle. MTO1 is implicated in expression of aminoglycoside-induced and non-syndromic deafness phenotypes associated with mutations in 12S rRNA gene, indicating potential involvement in pathogenesis of these hearing disorders. Additionally, MTO1 exists as multiple isoforms produced by alternative splicing, which may contribute to diverse functional roles in cellular metabolism and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MTO1 Antibody (H-5) References:
- Isolation and characterization of the putative nuclear modifier gene MTO1 involved in the pathogenesis of deafness-associated mitochondrial 12 S rRNA A1555G mutation. | Li, X., et al. 2002. J Biol Chem. 277: 27256-64. PMID: 12011058
- A human mitochondrial GTP binding protein related to tRNA modification may modulate phenotypic expression of the deafness-associated mitochondrial 12S rRNA mutation. | Li, X. and Guan, MX. 2002. Mol Cell Biol. 22: 7701-11. PMID: 12370316
- Identification and characterization of mouse MTO1 gene related to mitochondrial tRNA modification. | Li, R., et al. 2003. Biochim Biophys Acta. 1629: 53-9. PMID: 14522080
- Mitochondria-specific RNA-modifying enzymes responsible for the biosynthesis of the wobble base in mitochondrial tRNAs. Implications for the molecular pathogenesis of human mitochondrial diseases. | Umeda, N., et al. 2005. J Biol Chem. 280: 1613-24. PMID: 15509579
- Phenotype of non-syndromic deafness associated with the mitochondrial A1555G mutation is modulated by mitochondrial RNA modifying enzymes MTO1 and GTPBP3. | Bykhovskaya, Y., et al. 2004. Mol Genet Metab. 83: 199-206. PMID: 15542390
- MTO1 codes for a mitochondrial protein required for respiration in paromomycin-resistant mutants of Saccharomyces cerevisiae. | Colby, G., et al. 1998. J Biol Chem. 273: 27945-52. PMID: 9774408
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MTO1 Antibody (H-5) | sc-398386 | 200 µg/ml | $322.00 | |||
MTO1 Antibody (H-5): m-IgG Fc BP-HRP Bundle | sc-527768 | 200 µg Ab; 10 µg BP | $361.00 | |||
MTO1 Antibody (H-5): m-IgG1 BP-HRP Bundle | sc-533141 | 200 µg Ab; 20 µg BP | $361.00 | |||
MTO1 Antibody (H-5) AC | sc-398386 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MTO1 Antibody (H-5) HRP | sc-398386 HRP | 200 µg/ml | $322.00 | |||
MTO1 Antibody (H-5) FITC | sc-398386 FITC | 200 µg/ml | $336.00 | |||
MTO1 Antibody (H-5) PE | sc-398386 PE | 200 µg/ml | $349.00 | |||
MTO1 Antibody (H-5) Alexa Fluor® 488 | sc-398386 AF488 | 200 µg/ml | $364.00 | |||
MTO1 Antibody (H-5) Alexa Fluor® 546 | sc-398386 AF546 | 200 µg/ml | $364.00 | |||
MTO1 Antibody (H-5) Alexa Fluor® 594 | sc-398386 AF594 | 200 µg/ml | $364.00 | |||
MTO1 Antibody (H-5) Alexa Fluor® 647 | sc-398386 AF647 | 200 µg/ml | $364.00 | |||
MTO1 Antibody (H-5) Alexa Fluor® 680 | sc-398386 AF680 | 200 µg/ml | $364.00 | |||
MTO1 Antibody (H-5) Alexa Fluor® 790 | sc-398386 AF790 | 200 µg/ml | $364.00 | |||
MTO1 (H-5) Neutralizing Peptide | sc-398386 P | 100 µg/0.5 ml | $69.00 |