MCR Antibody (H10E4C9F): sc-53000
- MCR Antibody (H10E4C9F) is a mouse monoclonal IgG1 κ MCR antibody, cited in 4 publications, provided at 200 µg/ml
- raised against aldosterone 3 coupled to bovine serum albumin
- MCR Antibody (H10E4C9F) is recommended for detection of MCR of mouse, rat and human origin by WB, IP, IF and IHC(P)
- Anti-MCR Antibody (H10E4C9F) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for MCR Antibody (H10E4C9F) for WB and IHC(P) applications. These reagents are now offered in bundles with MCR Antibody (H10E4C9F) (see ordering information below).
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MCR Antibody (H10E4C9F) is a mouse monoclonal IgG1 kappa light chain antibody that detects MCR protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). MCR (H10E4C9F) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The mineralocorticoid receptor (MCR) plays a crucial role in the regulation of electrolyte balance and blood pressure by mediating the effects of mineralocorticoid hormones, such as aldosterone. Located primarily in epithelial tissues, MCR is essential for the transport of sodium, potassium, and hydrogen ions, which are vital for maintaining homeostasis. Upon binding to aldosterone, MCR activates gene transcription, leading to increased expression of proteins involved in ion transport. The regulation of MCR is complex, involving both negative autoregulation and positive autoregulation mechanisms, which ensure that MCR activity is finely tuned to physiological needs. Additionally, MCR interaction with heat shock protein 90 (HSP90) is critical for proper function, as this association is necessary for hormone binding and subsequent receptor activation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MCR Antibody (H10E4C9F) References:
- Cellular mechanisms of action of mineralocorticoid hormones. | Johnson, JP. 1992. Pharmacol Ther. 53: 1-29. PMID: 1322539
- Cloning of human mineralocorticoid receptor complementary DNA: structural and functional kinship with the glucocorticoid receptor. | Arriza, JL., et al. 1987. Science. 237: 268-75. PMID: 3037703
- Mineralocorticoid Receptor Activation and Atrial Fibrosis. | Brown, NJ. 2019. Hypertension. 73: 294-295. PMID: 30595119
- Antihypertensive power of Naringenin is mediated via attenuation of mineralocorticoid receptor (MCR)/ angiotensin converting enzyme (ACE)/ kidney injury molecule (Kim-1) signaling pathway. | Oyagbemi, AA., et al. 2020. Eur J Pharmacol. 880: 173142. PMID: 32422184
- Mineralocorticoid Receptor Antagonists for Diabetic Kidney Disease. | Rossing, P. 2020. Clin J Am Soc Nephrol. 15: 1696-1698. PMID: 33239412
- Mineralocorticoid receptor antagonists in diabetic kidney disease - mechanistic and therapeutic effects. | Barrera-Chimal, J., et al. 2022. Nat Rev Nephrol. 18: 56-70. PMID: 34675379
- Mineralocorticoid Receptor Antagonism by Finerenone Attenuates Established Pulmonary Hypertension in Rats. | Tu, L., et al. 2022. Hypertension. 79: 2262-2273. PMID: 35979822
- Mineralocorticoid receptor knockout in Schwann cells alters myelin sheath thickness. | González-Mayoral, A., et al. 2023. J Endocrinol. 258: PMID: 37195271
- Autoregulation of corticosteroid receptors. How, when, where, and why? | Schmidt, TJ. and Meyer, AS. 1994. Receptor. 4: 229-57. PMID: 7894339
- Inherited forms of mineralocorticoid hypertension. | White, PC. 1996. Hypertension. 28: 927-36. PMID: 8952579
- Inhibition of mineralocorticoid and glucocorticoid receptor function by the heat shock protein 90-binding agent geldanamycin. | Bamberger, CM., et al. 1997. Mol Cell Endocrinol. 131: 233-40. PMID: 9296382
- Transcriptional regulation of the steroid receptor genes. | Kumar, MV. and Tindall, DJ. 1998. Prog Nucleic Acid Res Mol Biol. 59: 289-306. PMID: 9427846
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MCR Antibody (H10E4C9F) | sc-53000 | 200 µg/ml | $322.00 | |||
MCR Antibody (H10E4C9F): m-IgG Fc BP-HRP Bundle | sc-526757 | 200 µg Ab; 10 µg BP | $361.00 | |||
MCR Antibody (H10E4C9F): m-IgG1 BP-HRP Bundle | sc-532130 | 200 µg Ab; 20 µg BP | $361.00 | |||
MCR Antibody (H10E4C9F) AC | sc-53000 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MCR Antibody (H10E4C9F) HRP | sc-53000 HRP | 200 µg/ml | $322.00 | |||
MCR Antibody (H10E4C9F) FITC | sc-53000 FITC | 200 µg/ml | $336.00 | |||
MCR Antibody (H10E4C9F) PE | sc-53000 PE | 200 µg/ml | $349.00 | |||
MCR Antibody (H10E4C9F) Alexa Fluor® 488 | sc-53000 AF488 | 200 µg/ml | $364.00 | |||
MCR Antibody (H10E4C9F) Alexa Fluor® 546 | sc-53000 AF546 | 200 µg/ml | $364.00 | |||
MCR Antibody (H10E4C9F) Alexa Fluor® 594 | sc-53000 AF594 | 200 µg/ml | $364.00 | |||
MCR Antibody (H10E4C9F) Alexa Fluor® 647 | sc-53000 AF647 | 200 µg/ml | $364.00 | |||
MCR Antibody (H10E4C9F) Alexa Fluor® 680 | sc-53000 AF680 | 200 µg/ml | $364.00 | |||
MCR Antibody (H10E4C9F) Alexa Fluor® 790 | sc-53000 AF790 | 200 µg/ml | $364.00 |