Lyn Double Nickase Plasmid (h): sc-400463-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Lyn Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Lyn Double Nickase Plasmid (h) and Lyn Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LYN. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Lyn Antibody (H-6): sc-7274
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Lyn Double Nickase Plasmid (h) | sc-400463-NIC | 20 µg | $410.00 | |||
Lyn Double Nickase Plasmid (h2) | sc-400463-NIC-2 | 20 µg | $410.00 |
Human LYN encodes Lyn, a Src family non-receptor tyrosine kinase that couples immunoreceptor and cytokine receptor signaling to downstream pathways controlling proliferation, survival, adhesion, and cytoskeletal remodeling. Lyn phosphorylates ITAM-containing adaptor proteins and modulates signaling through B cell receptor, Fc receptor, and integrin networks, intersecting with PI3K–AKT, MAPK, and NF-κB cascades. In hematopoietic lineages, Lyn also functions in feedback control of activation thresholds via inhibitory receptors and phosphatases, shaping innate and adaptive immune responses. Dysregulated LYN activity or expression has been associated with aberrant inflammatory signaling and altered growth signaling in multiple disease contexts, supporting its use as a mechanistic node in immunology and cancer biology research.
Lyn Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LYN locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LYN. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LYN function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LYN-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.